Determination of neutrophil Fcc receptor IIIb antigens (HNA-1a, HNA-1b and HNA-...

• Author(s): Costa, Elísio ; Antunes, Marika Bini ; Faria, Susana ; Vieira, Emília ; Branca, Rosa ; Barbot, José ; Santos, Rosário
• Date: 2004
• Persistent ID: http://hdl.handle.net/10198/507
• Origin: Biblioteca Digital do IPB
• Subject(s): FccRIIIB antigens,; Neutrophil
• Document Type: Article
• Language: English

Description

We evaluate a technique for genotyping HNA-1a, -1b and -1c antigens, resorting to fluorescence-primed allele-specific polymerase chain reaction (FPAS-PCR), and determine the frequency of the different genotypes in a normal Portuguese population. Our results indicate that the FPAS-PCR system is a reliable and simple tool for genotyping the neutrophil Fcc receptor IIIB antigens. The HNA-1a, -1b and -1c gene frequencies of 42.98, 84.21 and 6.14%, respectively, found in this study are similar to those reported for other white populations.