Selection and characterization of a multivalent Salmonella phage and its produc...

• Author(s): Santos, Sílvio ; Fernandes, E. ; Carvalho, Carla M. ; Sillankorva, Sanna ; Krylov, V. N. ; Pleteneva, E. A. ; Shaburova, O. V. ; Nicolau, Ana ; Ferreira, E. C. ; Azeredo, Joana
• Date: 2010
• Persistent ID: http://hdl.handle.net/1822/11462
• Origin: RepositóriUM - Universidade do Minho
• Document Type: Article
• Language: English

Description

We report the selection and amplification of the broad-host-range Salmonella phage phi PVP-SE1 in an alternative nonpathogenic host. The lytic spectrum and the phage DNA restriction profile were not modified upon replication in Escherichia coli Bl21, suggesting the possibility of producing this phage in a nonpathogenic host, contributing to the safety and easier approval of a product based on this Salmonella biocontrol agent.