Encontrados 47 documentos, a visualizar página 1 de 5
Evaluation of the ability of C. albicans to form biofilm in the presence of pha...
Pires, Diana; Silva, Sónia Carina; Almeida, Carina; Henriques, Mariana; Anderson, Erin M.; Lam, Joseph S.; Sillankorva, Sanna; Azeredo, JoanaPseudomonas aeruginosa and Candida albicans are disparate microbial species, but both are known to be opportunistic pathogens frequently associated with nosocomial infections. The aim of this study was to provide a better understanding of the interactions between these microorganisms in dual-species biofilms. Several bacteriophage-resistant P. aeruginosa phenotypes have been isolated and were used in dual-speci...
Genomic characterization of the Staphylococcus epidermidis-specific bacteriopha...
Melo, Luís D. R.; Oliveira, F.; Sillankorva, Sanna; Pérez-Cabezas, B.; Ackermann, H. W.; Kropinski, Andrew M.; Vilanova, Manuel; Azeredo, JoanaStaphylococcus epidermidis is one of the most frequent causative agents of nosocomial infections, predominantly in patients with indwelling medical devices. This microrganism may form biofilms which are microbial structures very tolerant to the host immune defenses and to antibiotherapy. Therefore, studies are needed in order to develop effective methods for biofilm control. Currently, bacteriophages (phages) a...
Bacteriophages and their role in food safety
Sillankorva, Sanna; Oliveira, Hugo; Azeredo, JoanaThe interest for natural antimicrobial compounds has increased due to alterations in consumer positions towards the use of chemical preservatives in foodstuff and food processing surfaces. Bacteriophages fit in the class of natural antimicrobial and their effectiveness in controlling bacterial pathogens in agro-food industry has led to the development of different phage products already approved by USFDA and US...
Genome sequence of the broad-host-range Pseudomonas phage Phi-S1
Sillankorva, Sanna; Kropinski, Andrew M.; Azeredo, JoanaThe broad-host-range lytic Pseudomonas phage Φ-S1 possess a 40,192 bp double-stranded DNA (dsDNA) genome of 47 open reading frames (ORFs) and belongs to the family Podoviridae, subfamily Autographivirinae, genus T7likevirus.
Complete genome sequence of the lytic Pseudomonas fluorescens phage ϕIBB-PF7A
Sillankorva, Sanna; Kluskens, Leon; Linghorn, E.; Kropinski, Andrew M.; Neubauer, P.; Azeredo, JoanaBackground Phage ϕIBB-PF7A is a T7-like bacteriophage capable of infecting several Pseudomonas fluorescens dairy isolates and is extremely efficient in lysing this bacterium even when growing in biofilms attached to surfaces. This work describes the complete genome sequence of this phage. Results The genome consists of a linear double-stranded DNA of 40,973 bp, with 985 bp long direct terminal repeats and a G...
The ability of C. albicans to form biofilm in the presence of sensitive and pha...
Pires, Diana; Silva, Sónia Carina; Henriques, Mariana; Sillankorva, Sanna; Azeredo, JoanaP. aeruginosa and C. albicans are human opportunistic pathogens frequently associated with nosocomial infections. Pathogenic interactions between them have already been identified and it has been reported that C. albicans’ morphology and virulence are significantly affected by the presence of P. aeruginosa. In this work, the interaction between these 2 microorganisms in mixed biofilms was studied. The behavior ...
Efficacy studies of phage phiIBB-Sep1 against S. epidermidis cells under differ...
Melo, Luís D. R.; Oliveira, F.; Sillankorva, Sanna; Pérez-Cabezas, B.; Vilanova, Manuel; Azeredo, Joana; Cerca, NunoStaphylococcus epidermidis has recently become known as a usual cause of nosocomial infections, predominantly in patients with indwelling medical devices. Although, S. epidermidis infections only rarely develop into life-threatening diseases, they are very frequent and difficult to treat due to the ability of this bacterium to adhere to the surfaces of indwelling medical devices and form biofilms. When S. epide...
Combined antibiotic-phage therapies to control Pseudomonas aeruginosa biofilms
Sillankorva, Sanna; Rodrigues, A. C.; Azeredo, JoanaPseudomonas aeruginosa is responsible for 65% of mortality in hospitals all over the world. Its prevalence is attributed to factors such as: intrinsic resistance determined by virulence factors; acquired resistance mechanisms that lead to a low susceptibility to antimicrobial agents; and ability to attach to any natural and artificial surfaces and form biofilms. The emergence of new strategies to control P. aer...
Can bacteriofages be effective in controlling harmful biofilms?
Sillankorva, Sanna; Pires, Diana; Melo, Luís D. R.; Cerca, Nuno; Azeredo, Joana(Bacterio)phages are viruses that specifically infect bacteria, causing cell lysis and therefore can be considered a valuable strategy for bacterial control. Recent studies have demonstrated the potential of using phages to control bacterial biofilms. Phages are able to penetrate the extracellular matrnc and can cause up to 90% of biofilm mass reduction even in old biofilms. However phage action can be impaired...
Efficacy of a broad host range lytic bacteriophage against E. coli adhered to u...
Sillankorva, Sanna; Oliveira, D.; Moura, A.; Henriques, Mariana; Faustino, A.; Nicolau, Ana; Azeredo, JoanaPersistent urinary tract infections (UTI) are often caused by E. coli adhered to urothelium. This type of cells is generally recognized as very tolerant to antibiotics which renders difficult the treatment of chronic UTI. This work investigates the use of lytic bacteriophages as alternative antimicrobial agents, particularly the interaction of phages with E. coli adhered to urothelium and specifically determine...
| Financiadores do RCAAP | |||||||
|
|
|
|
|
|
||