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A suggested classification for two groups of Campylobacter myoviruses

Most Campylobacter bacteriophages isolated to date have long contractile tails and belong to the family Myoviridae. Based on their morphology, genome size and endonuclease restriction profile, Campylobacter phages were originally divided into three groups. The recent genome sequencing of seven virulent campylophages reveal further details of the relationships between these phages at the genome organization leve...

The genome and proteome of a Campylobacter coli bacteriophage vB_CcoM-IBB_35 re...

Campylobacter is the leading cause of foodborne diseases worldwide. Bacteriophages (phages) are naturally occurring predators of bacteria, ubiquitous in the environment, with high host specificity and thus considered an appealing option to control bacterial pathogens. Nevertheless for an effective use of phages as antimicrobial agents, it is important to understand phage biology which renders crucial the analys...

Phages as therapeutic tools to control major foodborne pathogens : campylobacte...

Exploiting the biotechnological potential of bacteriophages towards food safety

Genomic and proteomic characterization of the broad host range Salmonella phage...

(Bacterio)phage PVP-SE1, isolated from a German wastewater plant, presents a high potential value as a biocontrol agent and as a diagnostic tool, even compared to the well-studied typing phage Felix 01, due to its broad lytic spectrum against different Salmonella strains. Sequence analysis of its genome (145,964 bp) shows it to be terminally redundant and circularly permuted. Its G C content, 45.6 mol%, is lowe...

Selection and characterization of a multivalent Salmonella phage and its produc...

We report the selection and amplification of the broad-host-range Salmonella phage phi PVP-SE1 in an alternative nonpathogenic host. The lytic spectrum and the phage DNA restriction profile were not modified upon replication in Escherichia coli Bl21, suggesting the possibility of producing this phage in a nonpathogenic host, contributing to the safety and easier approval of a product based on this Salmonella bi...

Genome sequence of the broad host range salmonella bacteriophage phi PVP-SE1

Salmonella is an important zoonotic pathogen and remains the primary cause of reported food poisoning worldwide with massive outbreaks. The increased resistance of Salmonella to antibiotics and other biocides has encouraged the development of alternatives to chemotherapy such as phage therapy. Phage phi PVP-SE1 is a salmonella phage isolated from a Germany (Regensburg) wastewater plant. This phage is characteri...

The use of antibiotics to improve phage detection and enumeration by the double...

Background The Double-Layer Agar (DLA) technique is extensively used in phage research to enumerate and identify phages and to isolate mutants and new phages. Many phages form large and well-defined plaques that are easily observed so that they can be enumerated when plated by the DLA technique. However, some give rise to small and turbid plaques that are very difficult to detect and count. To overcome these pr...