Author(s):
Longatto Filho, Adhemar 
; Etlinger, Daniela ; Pereira, Sónia Maria Miranda ; Kanamura, Cristina Takami ; Loreto, Celso di ; Santos, Gilda da Cunha ; Makabe, Sérgio ; Marques, José A. ; Santoro, Carmen L. F. ; Dôres, Gerson B. das ; Castelo, A.
Date: 2007
Persistent ID: http://hdl.handle.net/1822/5969
Origin: RepositóriUM - Universidade do Minho
Subject(s): P16; Cervical cancer; FHIT; HPV; Líquid-based cytology
Description
Objective. This cross-sectional study was intended to assess the association between immunohistochemical analysis of p16INK4A and fragile histidine triad (FHIT) and
the presence of precancerous cervical lesions.
Materials and Methods. Women seen at Pe´ rola Byington Hospital, São Paulo, Brazil, with histologically confirmed cervicitis (n = 31), cervical intraepithelial neoplasia (CIN) 1 (n = 30), CIN 2,3 (n = 30), and cervical cancer (n = 7) had also cervical material collected for liquid-based cytology, human papillomavirus Hybrid Capture 2 (HC2) test, and p16 and FHIT immunohistochemical reactions.
Results. p16 and FHIT reactions were scored as the following: G1%, 1% to 5%, 95% to 25%, and 925%.
Receiver operating curve analysis was used to select p16 and FHIT score cutoffs for further categorical analyses. All but one of the 37 CIN 2,3/cancer cases had a p16 score of greater than 1% to 5%. Among the 61 cervicitis/CIN 1 cases, 46 (75%) had a p16 score lower than 1% to 5%. In contrast, no association of FHIT expression and severity of
cervical lesions could be demonstrated in this data set.
Receiver operating curve analyses suggested the score of 1% to 5% for p16 as the cutoff that best discriminates CIN 2,3/cancer from cervicitis/CIN 1. No cutoff for FHIT scores could be suggested with data set.
Conclusions. p16, but not FHIT expression, has the potential to be used as complementary diagnostic tool to investigate human papillomavirusYinduced cervical
lesions, if these results are confirmed in larger studies.
