Author(s):
Moreira, M. A. R. 
; Longatto Filho, Adhemar ; Taromaru, Eliane ; Queiroz, Geraldo Silva ; Jubé, L. F. ; Pinto, S. A. ; Schmitt, Fernando C.
Date: 2006
Persistent ID: http://hdl.handle.net/1822/5782
Origin: RepositóriUM - Universidade do Minho
Subject(s): Adenocarcinoma; Cervical intraepithelial lesion; HPV; Hybrid capture II; Squamous cells carcinoma
Description
Hybrid capture is an easy and highly sensitive technique for screening population due to its capacity to detect malignant and premalignant lesions of the cervix. To evaluate its ensitivity, we investigated the frequency of high-risk human papillomavirus (HPV) infection and its correlation with glandular malignant lesions, analyzing a total of 113 cases of adenocarcinomas and related lesions. High-risk HPV was investigated
using a hybrid capture II (HC2) assay. Samples were collected in two different ways: either brushed
directly from surgical specimens before fixation or collected from the patients. We also investigated the frequency
of HPV in squamous malignant lesions, 65 squamous cell carcinomas (SCC) and 66 in situ squamous cell carcinomas (ISSCC), to compare the occurrence of HPV for these lesions. The 113 glandular lesions comprised 62 invasive adenocarcinomas (IAC), 8 in situ adenocarcinomas (ISAC), 26 IAC plus SCC,
and 17 adenosquamous cells carcinomas (ASCC). The HPV-positive reactions were as follows: 51 (82.2%) in IAC, 8 (100%) in ISAC, 25 (96.1%) in IAC plus SCC, and 14 (82.3%) in ASCC. HC2-positive results in the squamous malignant lesions were as follows: 58 of 63 (89.0%) for SCC and 94 of 103 (91.2%) for ISSCC.
High-risk HPV infection was quite similar for glandular and pure squamous invasive malignant lesions, 82.2% and 89.0%, respectively, indicating that high-risk HPV is also highly prevalent in glandular lesions.
Although hybrid capture proved to be an excellent adjunctive technique, we do not believe its results merit replacing the Pap smear as a screening tool.
