Performance of the DNA-citoliq liquid-based cytology system compared with conve...

• Author(s): Alves, Venâncio Avancini Ferreira ; Castelo, A. ; Filho, A. L. ; Vianna, M. R. ; Taromaru, Eliane ; Namiyama, Gislene ; Lörincz, A. ; Dôres, Gerson B. das
• Date: 2006
• Persistent ID: http://hdl.handle.net/1822/5751
• Origin: RepositóriUM - Universidade do Minho
• Subject(s): Liquid-based cytology; DNA-Citoliq system; Thin layer cytology; Split-sample study; Severe
• Document Type: Article
• Language: English

Description

To evaluate the performance of a new, manual, simplified liquid-based system, DNA-Citoliq (Digene Brasil), employed under routine conditions as compared to conventional smears collected from six collaborating private laboratories. Methods: A panel of cytopathologists, who served as the gold standard diagnosis, adjudicated discordant opinions. Results: Of 3206 pairs of slides considered valid for comparison, there were 3008 in full agreement (93.8%), 112 (3.5%) with one diagnostic category discrepancies, and 86 (2.7%) discordant cases. Among the 288 borderline+ by either method, DNA-Citoliq detected abnormalities in 243 (84.4%), and conventional smears (CS) detected abnormalities in 178 (61.8%) (McNemar test, P < 0.000), a 36.5% increased detection of borderline+ cases. Conclusions: For mild dyskaryosis, DNA-Citoliq detected 176 cases and CS 125 cases (McNemar test, P < 0.000); and for moderate+severe dyskaryosis 66 versus 32 cases respectively (McNemar test, P < 0.000).