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Performance of the DNA-citoliq liquid-based cytology system compared with conve...
Alves, Venâncio Avancini Ferreira; Castelo, A.; Filho, A. L.; Vianna, M. R.; Taromaru, Eliane; Namiyama, Gislene; Lörincz, A.; Dôres, Gerson B. dasTo evaluate the performance of a new, manual, simplified liquid-based system, DNA-Citoliq (Digene Brasil), employed under routine conditions as compared to conventional smears collected from six collaborating private laboratories. Methods: A panel of cytopathologists, who served as the gold standard diagnosis, adjudicated discordant opinions. Results: Of 3206 pairs of slides considered valid for comparison, the...
Sistema DNA-Citoliq (DCS) : um novo sistema para citologia em base líquida : as...
Longatto Filho, Adhemar; Namiyama, Gislene; Castelo Filho, Adauto; Viann, Maria Regina; Dôres, Gerson B. das; Taromaru, ElianeIntroduction: DCS is a new liquid-base cytology system for cell collected in UCM. Objective: assess technical aspects of DCS under different pro-tocols. Methods: the first experiment was performed with 144 samples. After cervical scraping with Ayre spatula and endocervical brushing, the sample was immediately smeared on the slide and alcohol-fixed. The same brush with residual cells was used to scrap the ectoce...
Immunocytochemical expression of p16 INK4A and Ki-67 in cytologically negative ...
Longatto Filho, Adhemar; Utagawa, Maria Lúcia; Shirata, Neuza Kasumi; Pereira, Sónia Maria Miranda; Namiyama, Gislene; Kanamura, Cristina TakamiThis study was designed to analyze the cross-sectional comparison of the p16 INK4A and Ki-67 immunocytochemical expression in negative and equivocal (atyp-ical squamous cells of undetermined significance (ASC-US)) liquid-based cytology (LBC) samples testing positive for high-risk human papillomavirus (HPV) types with HC2 assay or polymerase-chain reaction (PCR). A series of 199 consecutive LBC speci-mens derive...
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