Decalactone production by Yarrowia lipolytica under increased O2 transfer rates

• Autor(es): Aguedo, Mário ; Gomes, Nelma ; Escamilla Garcia, Erandi ; Waché, Yves ; Mota, M. ; Teixeira, J. A. ; Belo, Isabel
• Data: 2005
• Identificador Persistente: http://hdl.handle.net/1822/3646
• Origem: RepositóriUM - Universidade do Minho
• Assunto(s): B-oxidation; Decalactones; O2; Pressure; Yarrowia lipolytica
• Tipo de Documento: Artigo
• Idioma: Inglês

Descrição

Yarrowia lipolytica converts methyl ricinoleate to γ-decalactone, a high-value fruity aroma compound. The highest amount of 3-hydroxy-γ-decalactone produced by the yeast (263 mg lˉ¹) occurred by increasing the kLa up to 120 hˉ¹ at atmospheric pressure; above it, its concentration decreased, suggesting a predominance of the activity of 3- ydroxyacyl-CoA dehydrogenase. Cultures were grown under high-pressure, i.e., under increased O2 solubility, but, although growth was accelerated, γ-decalactone production decreased. However, by applying 0.5 MPa during growth and biotransformation gave increased oncentrations of dec-2-en-4-olide and dec-3-en-4-olide (70 mg lˉ¹).