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Lipase production by Aspergillus ibericus using olive mill wastewater

Olive mill wastewater (OMW) characteristics make it a suitable resource to be used as a microbial culture media to produce value-added compounds, such as enzymes. In this work, the ability of the novel species Aspergillus ibericus to discolor OMW and produce lipase was studied. An initial screening on plates containing an OMW-based agar medium and an emulsified olive oil/rhodamine-B agar medium was employed to ...

Comparison of Yarrowia lipolytica and Pichia pastoris cellular response to diff...

Yeast cells exposed to adverse conditions employ a number of defense mechanisms in order to respond effectively to the stress effects of reactive oxygen species. In this work, the cellular response of Yarrowia lipolytica and Pichia pastoris to the exposure to the ROSinducing agents’ paraquat, hydrogen peroxide, and increased air pressure was analyzed. Yeast cells at exponential phase were exposed for 3 h to 1 m...

Batch and fed-batch growth of Pichia pastoris under increased air pressure

Pichia pastoris CBS 2612 behavior under air pressures of 1 bar, 3 bar and 5 bar in culture media of glycerol (pure and crude) and methanol was studied. Generally, the increase in oxygen transfer rate due to the increase of total pressure improved cellular growth for all carbon sources and for batch and fed-batch processes with different feeding rate strategies. In batch cultures, 1.4-fold, 1.2-fold, and 1.5-fol...

Impact of lipase-mediated hydrolysis of castor oil on γ-decalactone production ...

γ-Decalactone is an industrially interesting peach-like aroma compound that can be produced biotechnologically through the biotransformation of ricinoleic acid. Castor oil (CO) is the raw material most used as the ricinoleic acid source. The effect of different CO concentrations on the γ-decalactone production by Yarrowia lipolytica was investigated in batch processing, and 30 g L−1 was found to be the optimal ...

Erratum to : Impact of lipase-mediated hydrolysis of castor oil on γ-decalacton...

Erratum to : Batch and fed-batch growth of Pichia pastoris under increased air ...

Valorisation of olive pomace by solid-state fermentation with Aspergillus speci...

Pollution by olive mill wastes is a crucial problem in Mediterranean area and their proper management and utilization is demanded. Olive pomace offers excellent properties to produce enzymes by solid-state fermentation (SSF) using filamentous fungi. Particularly for lipase production, since it has residual content of olive oil. The aim of this work was to optimize the production of lipase by Aspergillus ibericu...

Immobilization of Yarrowia lipolytica for aroma production from castor oil

The main aim of this study was to compare different materials for Y. lipolytica immobilization that could be used in the production of γ-decalactone (a peach-like aroma) in order to prevent the toxic effect both of the substrate and the aroma upon the cells. Therefore, cells adsorption onto pieces of methyl polymethacrylate and of DupUM® was studied and further used in the biotransformation of castor oil into γ...

Oxygen mass transfer rate in a pressurized lab-scale stirred bioreactor

Oxygen mass transfer from air to the liquid phase in bioreactors with aerobic cultures has long been a serious impairment to the productivity of various bioprocesses. An increase of the oxygen mass transfer rate (OTR) can be the key to overcome oxygen limitation. The influence of higher air pressure on OTR was measured and a significantly enhanced OTR could be obtained. The oxygen volumetric mass transfer coeff...

Increasing gamma-decalactone production from castor oil by Yarrowia lipolytica ...