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The use of olive mill wastewater by wild type Yarrowia lipolytica strains : med...

BACKGROUND: The aim of this work was to study the ability of two different wild type strains of the yeast Yarrowia lipolytica to grow on olive mill wastewater (OMW) and their potential to produce high-value products such as lipases. Factors that affect cellular growth and OMW degradation were studied, such as nitrogen supplementation, cells concentration and surfactant addition. RESULTS: Both strains, W29 and I...

Production of 3-hydroxy-γ-decalactone, the precursor of two decenolides with fl...

3-Hydroxy-γ-decalactone is the precursor of dec-2 and dec-3-en-4-olides which are valuable aroma compounds not yet produced. To promote the accumulation of this lactone, the yeast Yarrowia lipolytica was placed in different environmental conditions aiming at altering β-oxidation fluxes. The concentration of substrate, pH, aeration and dissolved oxygen level were modified. We observed an important accumulation a...

Olive mill wastewater valorization : use by non-conventional yeasts

The ability of the strains Yarrowia lipolytica and Candida rugosa to grow on Olive Mill Wastewater (OMW) based medium and to produce high-value compounds from OMW (such as enzymes), while degrading this waste, was tested. Factors affecting cellular growth and OMW degradation were also studied, such as OMW composition, dilution and supplementation. OMW collected from 3-phase olive mill of the north region of Por...

Interactions between bacterial surfaces and milk proteins, impact on food emuls...

Bacteria possess physicochemical surface properties such as hydrophobicity, Lewis acid/base and charge which are involved in physicochemical interactions between cells and interfaces. Moreover, food matrices are complex and heterogeneous media, with a microstructure depending on interactions between the components in media (van der Waals, electrostatic or structural forces, etc.). Despite the presence of bacter...

Development of bioprocesses based in biphasic media involving the yeast Yarrowi...

Yarrowia lipolytica has been intensively used in our research group to develop different bioprocesses based in the ability of this yeast to use hydrophobic substrates. Work has been focused in the optimization of the production of y-decalactone and lipase, and to degrade and detoxify olive oil wastewater producing simultaneously high-value compounds, such as enzymes. Studies of the oxygen mass transfer phenomen...

Use of castor oil for aroma production by the yeast yarrowia lipolytica : optim...

Oxygen mass transfer in a biphasic medium : influence on the biotransformation ...

In this work, an empirical correlation is proposed to describe kLα as a function of operating conditions (agitation and aeration rates) and of oil and surfactant volumetric fractions in a biotransformation medium, an oil-in-water dispersion. An interaction effect between the oil and the surfactant effects was found, since oil presence increased kLα in the absence of the surfactant but had an opposite effect whe...

Production of γ-decalactoned by the yeast Yarrowia lipolytica using different r...

Biodegradation of olive mill wastewater by non-conventional yeasts

Application of a novel oscillatory flow micro-bioreactor to the production of γ...

A novel micro-bioreactor based on the oscillatory flow technology was applied to the scale-down of the biotechnological production of γ-decalactone. A decrease up to 50% of the time required to obtain the maximum concentration of the compound was observed, when compared with other scaled-down platforms (stirred tank bioreactor or shake flask). A three-fold increase in γ-decalactone productivity was obtained by ...