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A lactate shuttle system between tumour and stromal cells is associated with po...

Background In a malignant tumour, cancer cells are embedded in stromal cells, namely cancer-associated fibroblasts (CAFs). These CAFs are now accepted as important players in cancer dynamics, being involved in tumour growth and progression. Although there are various reports on the interaction between tumour and stromal cells, the clinico-pathological significance of this cross-talk is still largely unknown. In...

Impact of lipase-mediated hydrolysis of castor oil on γ-decalactone production ...

γ-Decalactone is an industrially interesting peach-like aroma compound that can be produced biotechnologically through the biotransformation of ricinoleic acid. Castor oil (CO) is the raw material most used as the ricinoleic acid source. The effect of different CO concentrations on the γ-decalactone production by Yarrowia lipolytica was investigated in batch processing, and 30 g L−1 was found to be the optimal ...

Erratum to : Impact of lipase-mediated hydrolysis of castor oil on γ-decalacton...

Monocarboxylate transporter 2 (MCT2) as putative biomarker in prostate cancer

INTRODUCTION: Monocarboxylate transporter 2 (MCT2) is a transmembrane protein involved in the transport of monocarboxylates such as pyruvate and lactate. In a previous study we described overexpression of MCT2 in prostate carcinoma raising the hypothesis of using MCT2 as a possible biomarker in prostate cancer. With the present study we aimed to compare the pattern of expression of MCT2 and alpha-methylacyl-CoA...

Fed-batch versus batch cultures of Yarrowia lipolytica for γ-decalactone produc...

Constant medium feeding rate and intermittent fed-batch fermentation strategies were investigated aiming to increase the yields of γ-decalactone production by Yarrowia lipolytica, using methyl ricinoleate as substrate and ricinoleic acid source. The accumulation of another compound, 3-hydroxy-γ-decalactone, was also analyzed since it derives from the direct precursor of γ-decalactone thereby providing informati...

Lipase induction in Yarrowia lipolytica for castor oil hydrolysis and its effec...

γ-Decalactone is an aromatic compound of industrial interest, resulting from the biotransformation of ricinoleic acid, the major constituent of castor oil. In order to increase the availability of the substrate to the cells for the aroma production, castor oil previously hydrolyzed can be used. This hydrolysis may be promoted by enzymatic action, more specifically by lipases. In this work, the influence upon th...

Biotransformation of ricinoleic acid into g-decalactone by Yarrowia lipolytica ...

Doctoral Dissertation for PhD degree in Chemical and Biological Engineering ; The biotechnological production of γ-decalactone (a peach-like aroma compound) by biotransformation of ricinoleic acid carried out by microorganisms is an interesting process to produce the aroma with a “natural” label, which is valuable, considering the preference of consumers. Although there are many works described in the literatu...

Empirical modelling as an experimental approach to optimize lactone production

The biotransformation of ricinoleic acid, carried out by Yarrowia lipolytica, leads to the formation of gama-decalactone, a well-known peach-like aroma compound, interesting to produce and to use in the flavouring industry, reason why it is imperative to define the most appropriate conditions for its production. Thus, the aim of this work is the optimization of operating conditions for this lactone. However, as...

Oil-in-water emulsions characterization by laser granulometry and impact on γ-d...

Oil-in-water emulsions composed of methyl ricinoleate (MR) or castor oil (CO) as the organic phase, stabilized by Tween 80, are in the basis of the biotechnological production of gama-decalactone. Y. lipolytica was used due to its ability to grow on hydrophobic substrates and to carry out that biotransformation. The characterization of oil droplets size distribution by laser granulometry was performed under dif...

Optimization of a colorimetric assay for yeast lipase activity in complex systems

The present work describes a simple and sensitive spectrophotometric method based on the release of p-nitrophenyl butyrate (p-NPB) for the estimation of lipase activity. The method was optimized and validated in biphasic complex media containing methyl ricinoleate (MR) or olive mill wastewater (OMW), although it may be used for other biphasic media. Reaction mixtures containing substrate (2.63 mM p-NPB in sodiu...