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Production of 3-hydroxy-γ-decalactone, the precursor of two decenolides with fl...

Escamilla Garcia, Erandi; Aguedo, Mário; Gomes, Nelma; Choquet, Armelle; Belo, Isabel; Teixeira, J. A.; Belin, Jean-Marc; Waché, Yves

3-Hydroxy-γ-decalactone is the precursor of dec-2 and dec-3-en-4-olides which are valuable aroma compounds not yet produced. To promote the accumulation of this lactone, the yeast Yarrowia lipolytica was placed in different environmental conditions aiming at altering β-oxidation fluxes. The concentration of substrate, pH, aeration and dissolved oxygen level were modified. We observed an important accumulation a...


Decalactone production by Yarrowia lipolytica under increased O2 transfer rates

Aguedo, Mário; Gomes, Nelma; Escamilla Garcia, Erandi; Waché, Yves; Mota, M.; Teixeira, J. A.; Belo, Isabel

Yarrowia lipolytica converts methyl ricinoleate to γ-decalactone, a high-value fruity aroma compound. The highest amount of 3-hydroxy-γ-decalactone produced by the yeast (263 mg lˉ¹) occurred by increasing the kLa up to 120 hˉ¹ at atmospheric pressure; above it, its concentration decreased, suggesting a predominance of the activity of 3- ydroxyacyl-CoA dehydrogenase. Cultures were grown under high-pressure, i.e...


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