Biofilms formation by Candida tropicalis and Candida parapsilosis on an in vitr...

• Author(s): Silva, Sónia Carina ; Henriques, Mariana ; Oliveira, Rosário ; Azeredo, Joana ; Williams, David
• Date: 2011
• Persistent ID: http://hdl.handle.net/1822/25840
• Origin: RepositóriUM - Universidade do Minho
• Document Type: Conference Object
• Language: English

Description

The primary event in Candida infection is the colonization of host mucosal surfaces, often leading to the formation of biofilms. Most cases of candidosis have been attributed to Candida albicans, however, recently, non-Candida albicans Candida species have been identified as frequent pathogens of humans. Given the clinical importance of biofilm growth, the aim of this work was to study the biofilm formation by Candida tropicalis and Candida parapsi/osis on an in vitro oral epithelium. Methods: Candida tropicalis (n=?) and Candida parapsi/osis (n=?) strains originating from oral and vaginal mucosa and from the urinary tract were used. The biofilms were formed on commercially available reconstituted human oral epithelium (RHOE) during 12h and 24h . . Confocal laser scanner microscopy (CLSM) was used to investigate the biofilm formation ability and its effect on epithelium. Simultaneously, the levels of lactate dehydrogenase (LDH) released by the epithelial cell were also determined to assess the extension of tissue damage.