Description
Cases of massive skin loss upon injury or disease still represent a serious healthcare challenge worldwide, and despite the many alternatives
available, results are not yet satisfactory. Skin Tissue Engineering, especially now combined with Stem Cell Research, holds great
expectation towards an efficient and high quality skin regeneration. Human embryonic stem cells (hESCs) are quite attractive for this
purpose, as they possess both immunoprevileged features and unique self-renewal and differentiation potency, being able to provide
unlimited biological material. In this specific work, an innovative hydrogel-based model, together with hESCs-derived epidermal cells is
proposed for skin regeneration. hESCs were moved from Mouse embryonic fibroblasts (MEFs), cultured in a feeder-free system comprising
Matrigel and mTeSR media, and characterized by immunocytochemistry for pluripotency markers. After expansion, hESCs were
differentiated towards the epidermal lineage,by culturing them in Collagen IV coated dishes, and upon supplementation of both Retinoic
acid and bone morphogenetic protein 4. Keratin 18/14 positive cells were obtained, confirming the success of the used protocol.
Differentiated epidermal cells were purified and cultured onto the hydrogel. Following several culture methodologies optimization, hESCsderived
epidermal cells were able to adhere, proliferate and form a stratified epidermis-like structure demonstrating the potential and
expectation for Skin Regeneration.
; Silva, L. P. da 
