Encontrados 53 documentos, a visualizar página 1 de 6
Bioactive macro/micro porous silk fibroin/Nano-sized calcium phosphate scaffold...
Yan, Leping; Correia, Joana Silva; Correia, C.; Caridade, S. G.; Fernandes, E. M.; Sousa, R. A.; Mano, J. F.; Oliveira, Joaquim M.; Oliveira, A. L.Aim: The development of novel silk/nano-sized calcium phosphate (silk/nano-CaP) scaffolds with highly dispersed CaP nanoparticles in the silk fibroin (SF) matrix for bone tissue engineering. Materials & methods: Nano-CaP was incorporated in a concentrated aqueous SF solution (16 wt.%) by using an in situ synthesis method. The silk/nano-CaP scaffolds were then prepared through a combination of salt-leaching/ lyo...
Unleashing the potential of supercritical fluids for polymer processing in tiss...
Duarte, Ana Rita C.; Santo, Vitor E.; Alves, A.; Silva, S. S.; Moreira-Silva, Joana; Silva, Tiago H.; Marques, A. P.; Gomes, Manuela E.; Sousa, R. A.One of the major scientific challenges that tissue engineering and regenerative medicine (TERM) faces to move from benchtop to bedside regards biomaterials development, despite the latest advances in polymer processing technologies. A variety of scaffolds processing techniques have been developed and include solvent casting and particles leaching, compression molding and particle leaching, thermally induced pha...
Benefits of spine stabilization with biodegradable scaffolds in spinal cord inj...
Silva, Nuno; Sousa, R. A.; Fraga, J. S.; Fontes, Marco; Almeida, Hugo Leite; Cerqueira, R.; Almeida, Armando; Sousa, Nuno; Reis, R. L.; Salgado, A. J.Spine stabilization upon spinal cord injury (SCI) is a standard procedure in clinical practice, but rarely employed in experimental models. Moreover, the application of biodegradable biomaterials for this would come as an advantage as it would eliminate the presence of a nondegradable prosthesis within the vertebral bone. Therefore, in the present work, we propose the use of a new biodegradable device specifica...
New biotextiles for tissue engineering : development, characterization and in v...
Almeida, L. R.; Martins, A. R.; Fernandes, E. M.; Oliveira, Mariana B.; Correlo, V. M.; Pashkuleva, I.; Marques, A. P.; Ribeiro, Ana S.This work proposes biodegradable textile-based structures for tissue engineering applications. We describe the use of two polymers, polybutylene succinate (PBS) proposed as a viable multifilamentand silk fibroin (SF), to produce fibre-based finely tuned porous architectures by weft knitting. PBS is here proposed as a viable extruded multifilament fibre to be processed by a textile-based technology. A comparativ...
Silk-based 3D biotextiles support human adipose derived stem cells towards oste...
Ribeiro, V. P.; Ribeiro, Ana S.; Silva, Carla J.; Durães, Nelson F.; Bonifácio, Graça; Marques, A. P.; Sousa, R. A.; Oliveira, A. L.; Reis, R. L.Publicado em "Journal of Tissue Engineering and Regenerative Medicine", vol. 7, supp. 1 (2013) ; The present work aims at evaluating the potential of recently developed 3D silk-based biotextile structures to support hASCs adhesion, proliferation and osteogenic differentiation. The 3D spacer structures were processed by using a knitting technology. Two knitted silk layers were assembled and spaced by a monofila...
Interactions between Schwann and olfactory ensheathing cells with a starch/poly...
Silva, Nuno; Sousa, R. A.; Pires, Ana; Sousa, Nuno; Salgado, A. J.; Reis, R. L.Spinal cord injury (SCI) represents a major world health problem. Therefore it is urgent to develop novel strategies that can specifically target it. We have previously shown that the implantation of starch-based scaffolds (SPCL) aimed for spine stabilization on SCI animals leads to motor skills improvements. Therefore, we hypothesize that the combination of these scaffolds with relevant cell populations for SC...
Human embryonic stem cell-derivatives in a hydrogel-based skin model
Cerqueira, M. T.; Silva, L. P. da; Sousa, R. A.; Correlo, V. M.; Marques, A. P.; Reis, R. L.Cases of massive skin loss upon injury or disease still represent a serious healthcare challenge worldwide, and despite the many alternatives available, results are not yet satisfactory. Skin Tissue Engineering, especially now combined with Stem Cell Research, holds great expectation towards an efficient and high quality skin regeneration. Human embryonic stem cells (hESCs) are quite attractive for this purpose...
Suitability of silk-based 3D biotextiles seeded with human adipose-derived stem...
Ribeiro, V. P.; Martins, A. R.; Marques, A. P.; Bonifácio, G.; Oliveira, A. L.; Sousa, R. A.; Reis, R. L.Human Adipose-derived Stem Cells (hASCs) became an emerging possibility for tissue replacement therapies, such as bone tissue regeneration. Due to their osteogenic differentiation potential, easy isolation, expansion and in vitro proliferation, they have become a highly potential source of seed cells to be seeded in bone tissue engineering (TE) constructs and have demonstrated promising prospects in bone regene...
Development of macro/micro porous silk fibroin scaffolds with nano-sized calciu...
Yan, Leping; Correia, Cristina; Correia, Joana Silva; Caridade, S. G.; Fernandes, E. M.; Mano, J. F.; Sousa, R. A.; Oliveira, Joaquim M.Macro/micro porous silk scaffolds with nano-sized calcium phosphate (CaP) particles were developed for bone tissue engineering. Different amounts of nano-sized CaP particles 4, 8, 16 and 25% (CaP:silk fibroin, wt/wt) were generated into the highly concentrated aqueous silk fibroin solutions via an in-situ approach. Afterwards, the pure silk and silk/nano-CaP porous scaffolds were produced by a combination of sa...
Development and Characterization of a Novel Hybrid Tissue Engineering–Based Sca...
Silva, Nuno; Salgado, A. J.; Sousa, R. A.; Oliveira, João T.; Adriano, Pedro; Leite-Almeida, Hugo; Cerqueira, R.; Almeida, Armando; Mastronardi, F.Spinal cord injury (SCI) represents a significant health and social problem, and therefore it is vital to develop novel strategies that can specifically target it. In this context, the objective of the present work was to develop a new range of three-dimensional (3D) tubular structures aimed at inducing the regeneration within SCI sites. Up to six different 3D tubular structures were initially developed by rapi...
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