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Gellan gum-based hydrogel bilayered scaffolds for osteochondral tissue engineering
Pereira, D. R.; Canadas, Raphael F.; Correia, Joana Silva; Marques, A. P.; Reis, R. L.; Oliveira, Joaquim M.It has been shown that hydrogel bilayered scaffolds combining cartilage- and bone-like layers are most advantageous for treating osteochondral defects. In this study, it is proposed the use of low acyl gellan gum (LAGG) for developing bilayered hydrogel scaffolds for osteochondral tissue engineering. The cartilage-like layer of the GG-based bilayered hydrogel scaffolds is composed of LAGG (2 wt%). By adding a 2...
Human skin cell fractions fail to self-organize within a gellan gum/Hyaluronic ...
Cerqueira, M. T.; Silva, L. P. da; Santos, T. C.; Pirraco, Rogério; Correlo, V. M.; Marques, A. P.; Reis, R. L.Split-thickness autografts still are the current gold standard to treat skin, upon severe injuries. Nonetheless, autografts are dependent on donor site availability and often associated to poor quality neoskin. The generation of dermal–epidermal substitutes by tissue engineering is seen as a promising strategy to overcome this problematic. However, solutions that can be safely and conveniently transplanted in o...
Photocrosslinkable Kappa-carrageenan hydrogels for tissue engineering applications
Mihaila, Silvia M.; Gaharwar, Akhilesh K.; Reis, R. L.; Marques, A. P.; Gomes, Manuela E.; Khademhosseini, AliPublished online ; Kappa carrageenan ( κ -CA) is a natural-origin polymer that closely mimics the glycosaminoglycan structure, one of the most important constituents of native tissues extracellular matrix. Previously, it has been shown that κ -CA can crosslink via ionic interactions rendering strong, but brittle hydrogels. In this study, we introduce photocrosslinkable methacrylate moieties on the κ -CA backbo...
Effect of monocytes/macrophages on the early osteogenic differentiation of hBMSCs
Pirraco, R. P.; Reis, R. L.; Marques, A. P.Heterotypic cell interactions are essential for the homeostasis of bone tissue, in particular the widely studied interaction between osteoblasts and osteoclasts. Closely related with osteoclasts are monocytes/macrophages. These have been shown to produce osteogenic factors, e.g. BMP-2, which plays a key role in bone metabolism. However, the mechanisms through which monocytes/macrophages interact with osteoblast...
An investigation of the potential application of chitosan/aloe-based membranes ...
Silva, S. S.; Popa, Elena G.; Gomes, Manuela E.; Cerqueira, M. T.; Marques, A. P.; Caridade, S. G.; Teixeira, P.; Sousa, Cláudia; Mano, J. F.A significant number of therapeutics derived from natural polymers and plants have been developed to replace or to be used in conjunction with existing dressing products. The use of the therapeutic properties of aloe vera could be very useful in the creation of active wound dressing materials. The present work was undertaken to examine issues concerning structural features, topography, enzymatic degradation beh...
Unleashing the potential of supercritical fluids for polymer processing in tiss...
Duarte, Ana Rita C.; Santo, Vitor E.; Alves, A.; Silva, S. S.; Moreira-Silva, Joana; Silva, Tiago H.; Marques, A. P.; Gomes, Manuela E.; Sousa, R. A.One of the major scientific challenges that tissue engineering and regenerative medicine (TERM) faces to move from benchtop to bedside regards biomaterials development, despite the latest advances in polymer processing technologies. A variety of scaffolds processing techniques have been developed and include solvent casting and particles leaching, compression molding and particle leaching, thermally induced pha...
Human adipose stem cells cell sheet constructs impact epidermal morphogenesis i...
Cerqueira, M. T.; Pirraco, R. P.; Santos, T. C.; Rodrigues, D. B.; Frias, A. M.; Martins, A.; Reis, R. L.; Marques, A. P.Among the wide range of strategies to target skin repair/regeneration, tissue engineering (TE) with stem cells at the forefront, remains as the most promising route. Cell sheet (CS) engineering is herein proposed, taking advantage of particular cell-cell and cell-extracellular matrix (ECM) interactions and subsequent cellular milieu, to create 3D TE constructs to promote full-thickness skin wound regeneration. ...
Adipose stem cell-derived osteoblasts sustain the functionality of endothelial ...
Pirraco, Rogério; Ferreira, B. M.; Santos, T. C.; Frias, A. M.; Marques, A. P.; Reis, R. L.Vascularization is the most pressing issue in tissue engineering (TE) since ensuring that engineered constructs are adequately perfused after in vivo transplantation is essential for the construct’s survival. The combination of endothelial cells with current TE strategies seems the most promising approach but doubts persist as to which type of endothelial cells to use. Umbilical cord blood (UCB) cells have been...
VEGF and FGF-2 incorporation in starch-based bone tissue engineering constructs...
Santos, T. C.; Morton, Tatjana J.; Moritz, Martina; Pfeifer, Sabine; Reise, Kathrin; Marques, A. P.; Castro, António G.; Reis, R. L.The ideal bone tissue-engineered (TE) construct remains to be found, although daily discoveries significantly contribute to improvements in the field and certainly have valuable long-term outcomes. In this work, different TE elements, aiming at bone TE applications, were assembled and its effect on the expression of several vas- cularization/angiogenesis mediators analyzed. Starch/polycaprolactone (SPCL) scaffo...
Interfollicular epidermal stem cells : boosting and rescuing from adult skin
Cerqueira, M. T.; Frias, A. M.; Reis, R. L.; Marques, A. P.Publicado na série "Methods in Molecular Biology", vol. 989 (2013), ISSN 1064-3745 ; Epidermal stem cells isolation struggle remains, mainly due to the yet essential requirement of well-defined approaches and markers. The herein proposed methodology integrates an assemblage of strategies to accomplish the enrichment of the interfollicular epidermal stem cells multipotent fraction and their subsequent separatio...
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