Monoolein as helper lipid for non-viral transfection in mammals

• Author(s): Oliveira, A. C. N. ; Silva, J. P. Neves ; Coutinho, Paulo J. G. ; Gomes, A. A. ; Coutinho, O. P. ; Oliveira, M. Elisabete
• Date: 2010
• Persistent ID: http://hdl.handle.net/1822/14095
• Origin: RepositóriUM - Universidade do Minho
• Subject(s): Lipoplexes; Transfection; DODAB; Monoolein
• Document Type: Conference Object
• Language: English

Description

Lipoplexes composed of pDNA and DODAB/MO at different molar ratios (4:1, 2:1 and 1:1) and different cationic lipid/DNA charge ratios were investigated. The physicochemical properties of the lipoplexes (size and charge), the pDNA complexation, and the effect of heparin on pDNA release, were studied by Dynamic Light Scattering, Zeta Potential, and Ethidium Bromide exclusion assays. The cytotoxicity, transfection efficiency and the intracellular localization of DNA were evaluated on 293T cells.