The main objective of this work was to attain a deeper knowledge of contact lenses (CL) optical properties and to understand the influence of CL power on other properties which can affect the optical performance and compromise the ocular physiology.
The artificial introduction of nucleic acids (NA) into mammalian cells (transfection) has become, in recent years, a well-established procedure in basic and applied research, which allowed the study of gene function and regulation. The advances in this area have made possible the use of these methods for gene-based medicines, which constitute alternative therapeutic approaches. One of the most prominent methods...
The aim of this study is to analyze surface properties of different hydrogel and silicone-hydrogel contact lenses (CL) using Atomic Force Microscopy (AFM) Several commercial CL prepared by the same method of manufacture (cast-moulding), with different monomers composition, water content and surface treatment were used in this study. Surface roughness analysis was performed using AFM on Tapping® Mode using st...
Purpose To assess the disinfection efficacy of multipurpose solutions (MPS) against different bacterial species adhered either to silicon hydrogel or to conventional hydrogel contact lenses (CLs). The influences of the MPS formulation and the chemical composition of the lens material were investigated. Methods This investigation followed the standard 14729, which establishes the guidelines for assessing CL dis...
RICI4 - Book of Abstracts
Cationic liposomes/DNA (lipoplexes) have been widely used as non-viral vectors for transfection, the role of the neutral lipid in liposome formulation being determinant for the efficiency of this process [1,2]. In this work, we studied the potential of monoolein (MO) as helper lipid for cellular transfection. Lipoplexes composed of pDNA and dioctadecyldimethylammonium bromide (DODAB)/1-monooleoyl-rac-glycerol (...
The main objective of this work was to increase the retarding effect of the acid dye Telon Blue RR (C.I. Acid Blue 62) release on polyamide fibres dyeing by encapsulation of the dye in liposomes as an alternative to synthetic auxiliaries, in order to reduce effluent pollution. The retarding effect achieved with the use of mixed cationic liposomes of dioctadecyldimethylammonium bromide (DODAB)/soybean lecithin (...
Cationic liposomes/ DNA complexes (lipoplexes) have been widely used as nano-carriers for animal cell transfection, with the neutral lipid (helper) playing a determinant role for the efficiency of this process due to the formation of non-lammellar intermediates that are akin to membrane fusion process [1]. We have developed a novel formulation containing the cationic agent dioctadecyldimethylammonium bromide (D...
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