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Phase behavior and structural characterization of DODAB:MO lipid mixtures for d...

RICI4 - Book of Abstracts

Monoolein-based lipoplexes (DODAB/MO/DNA) as non-viral vector for transfection-...

Cationic liposomes/DNA (lipoplexes) have been widely used as non-viral vectors for transfection, the role of the neutral lipid in liposome formulation being determinant for the efficiency of this process [1,2]. In this work, we studied the potential of monoolein (MO) as helper lipid for cellular transfection. Lipoplexes composed of pDNA and dioctadecyldimethylammonium bromide (DODAB)/1-monooleoyl-rac-glycerol (...

DODAB/Monoolein mixed cationic liposomes for gene delivery : investigation by D...

A novel approach for the production of DODAB:MO lipoplexes : the influence of t...

Cationic liposomes/ DNA complexes (lipoplexes) have been widely used as nano-carriers for animal cell transfection, with the neutral lipid (helper) playing a determinant role for the efficiency of this process due to the formation of non-lammellar intermediates that are akin to membrane fusion process [1]. We have developed a novel formulation containing the cationic agent dioctadecyldimethylammonium bromide (D...

Monoolein as helper lipid for non-viral transfection in mammals

Lipoplexes composed of pDNA and DODAB/MO at different molar ratios (4:1, 2:1 and 1:1) and different cationic lipid/DNA charge ratios were investigated. The physicochemical properties of the lipoplexes (size and charge), the pDNA complexation, and the effect of heparin on pDNA release, were studied by Dynamic Light Scattering, Zeta Potential, and Ethidium Bromide exclusion assays. The cytotoxicity, transfection ...

Effects of preparation method on the physicochemical characteristics and cell t...

The success of gene therapy is decisively dependent on the development of effective carrier systems, able to compact and thus protect the genetic material as to avoid both in vitro and in vivo barriers for transgene delivery [1]. Given the problems of safety encountered with viral vectors, non-viral carrier systems are a safer alternative for the therapeutic delivery of genes [2]. Cationic liposomes/DNA (lipop...

Characterization of mixed DODAB/monoolein aggregates using Nile Red as a solvat...

The aim of this study was to get a further knowledge of the interactions between lamellar and non-lamellar forming-phospholipids, as a tool to better understand the membrane dynamics and function. We have studied the behaviour of aqueous mixtures of a cationic lipid (dioctadecyldimethylammonium bromide) and a neutral phospholipid (1-monoolein) in 10 to 150 micro molar total lipid concentration range. The effect...

Characterization of monoolein-based lipoplexes using fluorescence spectroscopy

Lipoplexes are commonly used as delivery systems in vitro and in vivo, the role of a neutral lipid as helper being of extreme importance in these systems. Cationic liposomes composed of dioctadecyldimethylammonium bromide (DODAB) with monoolein (MO) as a helper, at different molar ratios (1:2; 1:1 and 1:0.5) were prepared, and subsequently titrated to DNA. The structural and physicochemical properties of the li...