Cationic liposomes/DNA (lipoplexes) have been widely used as non-viral vectors for transfection, the role of the neutral lipid in liposome formulation being determinant for the efficiency of this process [1,2]. In this work, we studied the potential of monoolein (MO) as helper lipid for cellular transfection. Lipoplexes composed of pDNA and dioctadecyldimethylammonium bromide (DODAB)/1-monooleoyl-rac-glycerol (...
Lipoplexes composed of pDNA and DODAB/MO at different molar ratios (4:1, 2:1 and 1:1) and different cationic lipid/DNA charge ratios were investigated. The physicochemical properties of the lipoplexes (size and charge), the pDNA complexation, and the effect of heparin on pDNA release, were studied by Dynamic Light Scattering, Zeta Potential, and Ethidium Bromide exclusion assays. The cytotoxicity, transfection ...
The deep submarine valley near Setúbal that connects the continental platform to the Tagus Abyssal Plain is a submarine canyon of the type commonly knows as Gouf. In this article we try to show the tectonic influence in its formation and in the installation of its submarine hydrographic net. Some indirect evidences of erosive processes responsible for the formation of typical submarine forms of canyons are anal...
Introdução; Metodologia; Classificação climática; Classificação climática de Thornthwaite (1948); Classificação climática de Thornthwaite & Mather (1955); Conclusões. ; 2004
Introdução; Metodologia; Zoneamento de aptidão agroclimática; Zoneamento de risco climático; Indicações do Zoneamento de Aptidão Agroclimática e de Risco Climático; Zoneamento de aptidão agroclimática; Zoneamento de risco climático; Conclusões. ; 2004
O conhecimento da distribuição temporal e espacial dos principais elementos climáticos de uma região é de considerável relevância para os mais variados fins e aplicações, como o planejamento das atividades relacionadas à agricultura, à pecuária, à defesa civil e à preservação do meio ambiente, uma vez que possibilita uma melhor avaliação das disponibilidades climáticas da região em estudo. Este trabalho busca p...
The end stage situation, namely AIDS and malignant diseases, patients are analysed by the authors from the psychiatric and psychologic standpoint. A multidisciplinary clinical approach is pointed out and psychotherapeutic attitude and action parameters are referred to achieve both the understanding of persons in those situations, and the correct clinical praxis towards them. ; The end stage situation, namely A...
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