Encontrados 5 documentos, a visualizar página 1 de 1
Development of dioctadecyldimethylammonium bromide/monoolein liposomes for gene...
Silva, João P. N.; Oliveira, A. C. N.; Gomes, A. C.; Oliveira, M. ElisabeteThe artificial introduction of nucleic acids (NA) into mammalian cells (transfection) has become, in recent years, a well-established procedure in basic and applied research, which allowed the study of gene function and regulation. The advances in this area have made possible the use of these methods for gene-based medicines, which constitute alternative therapeutic approaches. One of the most prominent methods...
Monoolein-based lipoplexes (DODAB/MO/DNA) as non-viral vector for transfection-...
Oliveira, A. C. N.; Silva, J. P. Neves; Coutinho, Paulo J. G.; Gomes, A. A.; Coutinho, O. P.; Oliveira, M. ElisabeteCationic liposomes/DNA (lipoplexes) have been widely used as non-viral vectors for transfection, the role of the neutral lipid in liposome formulation being determinant for the efficiency of this process [1,2]. In this work, we studied the potential of monoolein (MO) as helper lipid for cellular transfection. Lipoplexes composed of pDNA and dioctadecyldimethylammonium bromide (DODAB)/1-monooleoyl-rac-glycerol (...
A novel approach for the production of DODAB:MO lipoplexes : the influence of t...
Oliveira, A. C. N.; Silva, J. P. Neves; Oliveira, I. M. S. C.; Coutinho, O. P.; Gomes, A. C.; Oliveira, M. ElisabeteCationic liposomes/ DNA complexes (lipoplexes) have been widely used as nano-carriers for animal cell transfection, with the neutral lipid (helper) playing a determinant role for the efficiency of this process due to the formation of non-lammellar intermediates that are akin to membrane fusion process [1]. We have developed a novel formulation containing the cationic agent dioctadecyldimethylammonium bromide (D...
Monoolein as helper lipid for non-viral transfection in mammals
Oliveira, A. C. N.; Silva, J. P. Neves; Coutinho, Paulo J. G.; Gomes, A. A.; Coutinho, O. P.; Oliveira, M. ElisabeteLipoplexes composed of pDNA and DODAB/MO at different molar ratios (4:1, 2:1 and 1:1) and different cationic lipid/DNA charge ratios were investigated. The physicochemical properties of the lipoplexes (size and charge), the pDNA complexation, and the effect of heparin on pDNA release, were studied by Dynamic Light Scattering, Zeta Potential, and Ethidium Bromide exclusion assays. The cytotoxicity, transfection ...
Effects of preparation method on the physicochemical characteristics and cell t...
Oliveira, A. C. N.; Silva, J. P. Neves; Coutinho, Paulo J. G.; Gomes, A. C.; Coutinho, O. P.; Oliveira, M. ElisabeteThe success of gene therapy is decisively dependent on the development of effective carrier systems, able to compact and thus protect the genetic material as to avoid both in vitro and in vivo barriers for transgene delivery [1]. Given the problems of safety encountered with viral vectors, non-viral carrier systems are a safer alternative for the therapeutic delivery of genes [2]. Cationic liposomes/DNA (lipop...
| Financiadores do RCAAP | |||||||
|
|
|
|
|
|
||