Monoolein as helper lipid for non-viral transfection in mammals

• Autor(es): Oliveira, A. C. N. ; Silva, J. P. Neves ; Coutinho, Paulo J. G. ; Gomes, A. A. ; Coutinho, O. P. ; Oliveira, M. Elisabete
• Data: 2010
• Identificador Persistente: http://hdl.handle.net/1822/14095
• Origem: RepositóriUM - Universidade do Minho
• Assunto(s): Lipoplexes; Transfection; DODAB; Monoolein
• Tipo de Documento: Documento de conferência
• Idioma: Inglês

Descrição

Lipoplexes composed of pDNA and DODAB/MO at different molar ratios (4:1, 2:1 and 1:1) and different cationic lipid/DNA charge ratios were investigated. The physicochemical properties of the lipoplexes (size and charge), the pDNA complexation, and the effect of heparin on pDNA release, were studied by Dynamic Light Scattering, Zeta Potential, and Ethidium Bromide exclusion assays. The cytotoxicity, transfection efficiency and the intracellular localization of DNA were evaluated on 293T cells.