Hypericum perforatum plant cells reduce Agrobacterium viability during co-culti...

• Author(s): Franklin, Gregory ; Conceição, Luís F. R. ; Kombrink, E. ; Dias, Alberto Carlos Pires
• Date: 2008
• Persistent ID: http://hdl.handle.net/1822/10495
• Origin: RepositóriUM - Universidade do Minho
• Subject(s): Agrobacterium; Hypericum; Plant defense response; Plant recalcitrance
• Document Type: Article
• Language: English

Description

Plant recalcitrance is the major barrier in developing Agrobacterium-mediated transformation protocols for several important plant species. Despite the substantial knowledge of T-DNA transfer process, very little is known about the factors leading to the plant recalcitrance. Here, we analyzed the basis of Hypericum perforatum L. (HP) recalcitrance to Agrobacterium-mediated transformation using cell suspension culture. When challenged with Agrobacterium, HP cells swiftly produced an intense oxidative burst, a typical reaction of plant defense. Agrobacterium viability started to decline and reached 99% mortality within 12 h, while the plant cells did not suffer apoptotic process. This is the Wrst evidence showing that the reduction of Agrobacterium viability during co-cultivation with recalcitrant plant cells can affect transformation.