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Study of Hypericum perforatum defense mechanisms against Colletotrichum gloeosp...
Conceição, Luís F. R.Tese de doutoramento em Ciências (ramo de conhecimento em Biologia) ; H. perforatum (HP) é uma planta medicinal encontrada em todo o mundo, sendo nativa da Europa, norte de África, Oriente Médio e grande parte da Ásia. Introduzida também noutras regiões do globo devido ao seu elevado valor terapêutico, essa planta é descrita desde tempos imemoriais para o tratamento de diversas maleitas. Actualmente, sua princ...
Xanthone biosynthesis in Hypericum perforatum cells provides antioxidant and an...
Franklin, Gregory; Conceição, Luís F. R.; Kombrink, E.; Dias, Alberto Carlos PiresXanthone production in Hypericum perforatum (HP) suspension cultures in response to elicitation by Agrobacterium tumefaciens co-cultivation has been studied. RNA blot analyses of HP cells co-cultivated with A. tumefaciens have shown a rapid up-regulation of genes encoding important enzymes of the general phenylpropanoid pathway (PAL, phenylalanine ammonia lyase and 4CL, 4-coumarate:CoA ligase) and xanthone bios...
Hypericum perforatum plant cells reduce Agrobacterium viability during co-culti...
Franklin, Gregory; Conceição, Luís F. R.; Kombrink, E.; Dias, Alberto Carlos PiresPlant recalcitrance is the major barrier in developing Agrobacterium-mediated transformation protocols for several important plant species. Despite the substantial knowledge of T-DNA transfer process, very little is known about the factors leading to the plant recalcitrance. Here, we analyzed the basis of Hypericum perforatum L. (HP) recalcitrance to Agrobacterium-mediated transformation using cell suspension c...
Induction of phenolic compounds in Hypericum perforatum L. cells by colletotric...
Conceição, Luís F. R.; Ferreres, Federico; Tavares, R. M.; Dias, Alberto Carlos PiresChanges in phenolic metabolism after elicitation with Colletotrichum gloeosporioides (CG) has been studied in Hypericum perforatum L. (HP) cell suspension cultures. Soluble phenolics were analysed by HPLC–DAD and HPLC–DAD–MS/MS. HP cultures elicited with the CG elicitor showed a significant increase in xanthone accumulation. Xanthone accumulation increased twelve fold when the cells were primed with methyl-jasm...
Hypericum perforatum cultures as a tool to study plant defence mechanisms again...
Conceição, Luís F. R.; Franklin, Gregory; Ribeiro, Catarina; Dias, Alberto Carlos PiresTo elucidate the defence mechanisms of Hypericum perforatum L. against Colletotrichum gloeosporioides, we utilized cell suspension cultures. When primed with methyl jasmonate, H. perforatum cells showed a double oxidative burst upon Colletotrichum gloeosporioides elicitation typical of a hypersensitive response. Phenolic profile of the cells was modified upon various stimuli such as salicylic acid, MeJ and C. g...
Changes in lignin content of Hypericum perforatum cells after Colletotrichum gl...
Conceição, Luís F. R.; Tavares, R. M.; Dias, Alberto Carlos Pires
Changes of metabolism in Hypericum perforatum cells after elicitation with Coll...
Conceição, Luís F. R.; Ribeiro, Catarina; Dias, Alberto Carlos PiresApresentação efectuada na 51th GA Meeting, Kiel, Germany, 2003.
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