Encontrados 11 documentos, a visualizar página 1 de 2
Signaling pathways influencing tumor microenvironment and their exploitation fo...
Sheeba, Caroline J.; Marslin, Gregory; Revina, Ann Mary; Franklin, GregoryIn the recent years, the “tumor microenvironment” has been receiving growing attention due to its involvement in neoplastic transformation, tumor growth, invasion and protection of tumor cells from host immune response. All these events are facilitated by chemical signals produced by the tumor as well as the surrounding stromal cells. This review is divided into two main parts in which the first part discusses ...
Hypericum sp. : essential oil composition and biological activities
Guedes, Ana P.; Franklin, Gregory; Ferreira, Manuel FernandesPhytochemical composition of Hypericum genus has been investigated for many years. In the recent past, studies on the essential oils (EO) of this genus have been progressing and many of them have reported interesting biological activities. Variations in the EO composition of Hypericum species influenced by seasonal variation, geographic distribution, phenological cycle and type of the organ in which EO are prod...
Elicitation of Hypericum perforatum cells with Agrobacterium tumefaciens induce...
Carvalho, A. C.; Franklin, Gregory; Dias, Alberto Carlos Pires; Lima, Cristóvão F.Hypericum perforatum (HP) commonly known as St. John’s wort is an important medicinal plant traditionally used in several ailments including mild to moderate depression. In a recent study, elicitation of HP cells (culture of cells in suspension) with Agrobacterium tumefaciens (AT) increased significantly the production of compounds with antioxidant and antimicrobial properties [1]. The ability of methanolic ext...
Hypericum perforatum cells accumulate anthocyanins and flavonoids at the expens...
Franklin, Gregory; Dias, Alberto Carlos PiresHypericum perforatum L. (HP) is well-known for its medicinal uses. Extracts of HP have been used in traditional medicine worldwide for several ailments (Franklin et al 2009). Pharmacological properties of HP extracts such as antidepressant, antioxidant, anti-inflammatory, hepatoprotective, and anticancer activities can be attributed to the phenolic compounds such as naphtodianthrones, xanthones, flavonoids etc....
Induction of antioxidant defenses in human HepG2 cells by a methanolic extract ...
Lima, Cristóvão F.; Carvalho, A. C.; Franklin, Gregory; Dias, Alberto Carlos PiresHypericum perforatum (HP) commonly known as St. John’s wort is an important medicinal plant traditionally used in several ailments including mild to moderate depression. In a recent study, elicitation of HP cells (culture of cells in suspension) with Agrobacterium tumefaciens (AT) increased significantly the production of compounds with antioxidant and antimicrobial properties [1]. The present investigation has...
Xanthone biosynthesis in Hypericum perforatum cells provides antioxidant and an...
Franklin, Gregory; Conceição, Luís F. R.; Kombrink, E.; Dias, Alberto Carlos PiresXanthone production in Hypericum perforatum (HP) suspension cultures in response to elicitation by Agrobacterium tumefaciens co-cultivation has been studied. RNA blot analyses of HP cells co-cultivated with A. tumefaciens have shown a rapid up-regulation of genes encoding important enzymes of the general phenylpropanoid pathway (PAL, phenylalanine ammonia lyase and 4CL, 4-coumarate:CoA ligase) and xanthone bios...
Hypericum perforatum plant cells reduce Agrobacterium viability during co-culti...
Franklin, Gregory; Conceição, Luís F. R.; Kombrink, E.; Dias, Alberto Carlos PiresPlant recalcitrance is the major barrier in developing Agrobacterium-mediated transformation protocols for several important plant species. Despite the substantial knowledge of T-DNA transfer process, very little is known about the factors leading to the plant recalcitrance. Here, we analyzed the basis of Hypericum perforatum L. (HP) recalcitrance to Agrobacterium-mediated transformation using cell suspension c...
Production of transgenic Hypericum perforatum plants via particle bombardment-m...
Franklin, Gregory; Oliveira, Margarida M.; Dias, Alberto Carlos PiresWe have developed particle bombardment-mediated transformation procedure for Hypericum perforatum L. (St. John’s wort), an important medicinal species that remains highly recalcitrant towards Agrobacterium-mediated transformation. Among the major transformation techniques evaluated in the present study (Agrobacterium tumefaciens-, A. rhizogenes- and biolistics-mediated), particle-bombardment-mediated gene trans...
Auxin pretreatment promotes regeneration of sugarcane (Saccharum spp. hybrids) ...
Franklin, Gregory; Arvinth, S.; Sheeba, Caroline J.; Kanchana, M.; Subramonian, N.We have developed a new, simple, quick and genotype-independent method for direct regeneration of sugarcane using novel midrib segment explants. Our protocol involves two steps: the pretreatment of starting material on MS (Murashige and Skoog (1962) Physiol Plant 15:473–497) medium containing 3.0 mg/l 2,4- dichlorophenoxyacetic acid (2,4-D) for 8 days under continuous dark and subsequent transfer of the explant...
Organogenesis and embryogenesis in several hypericum perforatum genotypes
Dias, Alberto Carlos Pires; Franklin, GregorySt John’s wort (Hypericum perforatum) is a valuable plant used as a herbal remedy or in phytopharmaceutical drugs to treat a variety of physical ailments. Much research has been performed to study the biochemical production of secondary metabolites of in vitro cultured plants or organs. However, all of these studies have looked at the regeneration of plants from explants in only one genotype. In addition, no st...
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