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Xanthone biosynthesis in Hypericum perforatum cells provides antioxidant and an...
Franklin, Gregory; Conceição, Luís F. R.; Kombrink, E.; Dias, Alberto Carlos PiresXanthone production in Hypericum perforatum (HP) suspension cultures in response to elicitation by Agrobacterium tumefaciens co-cultivation has been studied. RNA blot analyses of HP cells co-cultivated with A. tumefaciens have shown a rapid up-regulation of genes encoding important enzymes of the general phenylpropanoid pathway (PAL, phenylalanine ammonia lyase and 4CL, 4-coumarate:CoA ligase) and xanthone bios...
Hypericum perforatum plant cells reduce Agrobacterium viability during co-culti...
Franklin, Gregory; Conceição, Luís F. R.; Kombrink, E.; Dias, Alberto Carlos PiresPlant recalcitrance is the major barrier in developing Agrobacterium-mediated transformation protocols for several important plant species. Despite the substantial knowledge of T-DNA transfer process, very little is known about the factors leading to the plant recalcitrance. Here, we analyzed the basis of Hypericum perforatum L. (HP) recalcitrance to Agrobacterium-mediated transformation using cell suspension c...
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