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Phaeomoniella chlamydospora-induced oxidative burst in vitis vinifera cell susp...
Lima, Marta; Dias, Alberto Carlos PiresThe biphasic oxidative burst induced by Phaeomoniella chlamydospora extract (Pce) in Vitis vinifera (Vv) cell suspensions was investigated. Treatment of cell suspensions with diphenyleneiodonium chloride, an inhibitor of NADPH oxidase, prevented the Pce-induced biphasic reactive oxygen species (ROS) accumulation, suggesting that NADPH oxidase is the primary ROS source in the oxidative burst induced by Pce elici...
Response of vitis vinifera cell cultures to phaeomoniella chlamydospora : chang...
Lima, M. R. M.; Ferreres, F.; Dias, Alberto Carlos PiresCell suspension cultures of Vitis vinifera cv. Vinhão (Vv) were used to study the putative response of V. vinifera to Phaeomoniella chlamydospora (Pc), a fungus frequently associated with esca and grapevine decline. Cells were elicited with a Pc autoclaved biomass extract and methyl jasmonate (MeJ). Phenolic production was evaluated by HPLC-DAD and HPLC-MS/MS. Phenolic production of Vv cells significantly chang...
Analysis of DNA damage and repair in Saccharomyces cerevisiae using the comet a...
Azevedo, F.; Marques, Filipe; Fokt, Hanna; Domingues, Alberta; Dias, Alberto Carlos Pires; Johansson, Björn; Oliveira, Rui Pedro Soares deIn this work we used the model organism Saccharomyces cerevisiae to characterise the biological activity and the mechanism of action of phytochemicals. We have assessed DNA damage and repair using the comet assay, which displayed a dose-response relationship with different DNA-damaging agents. Subsequently, we used this system to assess the antigenotoxic properties of a leaf extract from Ginkgo biloba (GBE). Ex...
Elicitation of Hypericum perforatum cells with Agrobacterium tumefaciens induce...
Carvalho, A. C.; Franklin, Gregory; Dias, Alberto Carlos Pires; Lima, Cristóvão F.Hypericum perforatum (HP) commonly known as St. John’s wort is an important medicinal plant traditionally used in several ailments including mild to moderate depression. In a recent study, elicitation of HP cells (culture of cells in suspension) with Agrobacterium tumefaciens (AT) increased significantly the production of compounds with antioxidant and antimicrobial properties [1]. The ability of methanolic ext...
Evaluation of the protective effect of Hypericum perforatum phenolics compounds...
Vieira, Pedro Miguel de Sousa; Marques, Belém Sampaio; Ludovico, Paula; Dias, Alberto Carlos PiresParkinson’s disease (PD), first described by James Parkinson in 1817, is a chronic, progressive neurodegenerative disorder. The pathologic hallmark is a deterioration of the substantia nigra of yet unknown causes, resulting in a deficiency of dopamine, an important neurotransmitter for the basal ganglia circuit and the presence of cytoplasmic eosinophilic inclusions named Lewy ...
Hypericum perforatum phenolics compounds : protective role in the toxicity indu...
Vieira, Pedro Miguel de Sousa; Marques, Belém Sampaio; Ludovico, Paula; Dias, Alberto Carlos PiresParkinson’s disease (PD) is a neurodegenerative disorder with high prevalence and is characterized by the loss of dopaminergic neurons and the presence of cytoplasmic eosinophilic inclusions named Lewy bodies, in which α-synuclein is the major constituent. Several studies implicate abnormal protein accumulation, protein phosphorylation, mitochondrial dysfu...
Suppressing fatty acid uptake by a fatty acid secreting saccharomyces cerevisia...
Ribeiro, Gabriela; Côrte-Real, Manuela; Dias, Alberto Carlos Pires; Johansson, BjörnBiodiesel, in the form of fatty acid esters, produced by oleaginous microorganisms, could represent an attractive alternative of renewable liquid biofuels. Lipid metabolism has been studied extensively in S. cerevisiae and all genes encoding enzymes directly involved in lipid synthesis are known. In this model yeast, exogenous long-chain fatty acids are activated to coenzyme A derivatives prior to metabolic uti...
Delayed fatty acid uptake by a fatty acid secreting yeast strain with modified ...
Ribeiro, Gabriela; Côrte-Real, Manuela; Dias, Alberto Carlos Pires; Johansson, BjörnBiodiesel, in the form of fatty acid esters, produced by oleaginous microorganisms, could represent an attractive alternative of renewable liquid biofuels. In the model yeast S. Cerevisiae lipid metabolism has been studied extensively and all genes encoding enzymes directly involved in lipid synthesis are known. In this organism exogenous long-chain fatty acids are activated to coenzyme A derivatives prior to m...
Analysis of DNA damage and repair in saccharomyces cerevisiae using the comet a...
Azevedo, F.; Marques, Filipe; Fokt, Hanna; Domingues, Alberta; Dias, Alberto Carlos Pires; Johansson, Björn; Oliveira, Rui Pedro Soares deIn this work we used the model organism Saccharomyces cerevisiae to characterise the biological activity and the mechanism of action of phytochemicals. One of the goals is to use mutant strains affected in basic mechanisms of oxidative stress response and DNA repair in order to uncover the molecular targets of phytochemicals. We have assessed DNA damage and repair using the comet assay, evaluated as “comet tail...
Evaluation of antigenotoxic effects of phytochemicals : DNA protection from oxi...
Domingues, Alberta; Dias, Alberto Carlos Pires; Oliveira, Rui Pedro Soares deIn recent years, plant extracts and their phytochemicals have received increasing attention, especially in the field of pharmaceutical sciences and medicine, due to the potential prevention activity of a number of chronic and degenerative diseases including cancer and cardiovascular diseases. Most of the health-promoting effects have been attributed to antioxidant action including the ability to scavenge reacti...
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