Natural polymorphisms of HIV type 2 pol sequences from drug-naive individuals

• Author(s): Parreira, R. ; Monteiro, F. ; Pádua, E. ; Piedade, J. ; Venenno, T. ; Paixão, M.T. ; Esteves, A.
• Date: 2006
• Persistent ID: http://hdl.handle.net/10400.18/1587
• Origin: Repositório Científico do Instituto Nacional de Saúde
• Subject(s): HIV-2; Pol Sequences; Polymorphisms; Drug-naive; Portugal; Infecções Sexualmente Transmissíveis
• Document Type: Article
• Language: English

Description

Until today, the susceptibility of human immunodeficiency virus type 2 (HIV-2) to protease and nucleosidic reverse-transcriptase inhibitors (PI and NRTI, respectively) has not been clearly documented. In this report we studied HIV-2 proviral sequences (n = 30) from drug-naive patients. Our results revealed that several amino acid positions in the protease and reverse transcriptase coding sequence harbored residues that have been associated with drug resistance in HIV-1-infected patients. In particular, the M46I substitution in the protease was detected in 90% of the sequences analyzed, which, together with the other substitutions identified, may indicate a reduced susceptibility of HIV-2-infected drug-naive patients to PI. Furthermore, interpretation of genotypic data with four available algorithms, developed for interpretation of HIV-1 sequence data, suggested nonoverlapping profiles of drug resistance.