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Genetic Characterization of an Insect-Specific Flavivirus Isolated from Culex T...
Parreira, R; Cookc, S; Lopes, A; Matos, AP; Almeida, AP; Piedade, J; Esteves, AWe describe the full genetic characterization of an insect-specific flavivirus (ISF) from Culex theileri (Theobald) mosquitoes collected in Portugal. This represents the first isolation and full characterization of an ISF from Portuguese mosquitoes. The virus, designated CTFV, for Culex theileri flavivirus, was isolated in the C6/36 Stegomyia albopicta (=Aedes albopictus) cell line, and failed to replicate in v...
Fertilidade de quistos hidáticos em diferentes espécies de animais, em Portugal
Correia, V.; Beato, S.; Parreira, R.; Grácio, Maria A. A.A hidatidose apresenta uma distribuição cosmopolita e encontra-se entre as zoonoses de maior importância a nível mundial. O parasita Echinococcus granulosus, responsável por esta doença, apresenta uma distribuição cosmopolita afectando diversas espécies de animais e, acidentalmente, o Homem. O objectivo do estudo é a obtenção do estado larvar do E. granulosus (protoescólices) para o estudo da fertilidade dos qu...
Comparação de diferentes métodos de extracção de DNA em amostras de echinococcu...
Roque, C.; Beato, S.; Belo, J.; Parreira, R.; Grácio, M. A. A.O céstode E. granulosus, agente causal da hidatidose/equinococose, está presente em diversas espécies de animais e, ocidentalmente, em humanos. Este apresenta uma grande variabilidade genética, com dez genótipos diferentes descritos (G1 – G10) onde alguns destes já são considerados novas espécies. Estes genótipos encontram-se distribuídos um pouco por todo o mundo, inclusive Portugal. O presente trabalho tem co...
Hydatidosis in Portugal: a mitochondrial phylogeny approach
Beato, S.; Calado, M. M.; Parreira, R.; Grácio, M. A. A.Hydatidosis is a worldwide zoonosis caused by the larval stage of the tapeworm Echinococcus granulosus. The taxonomy and phylogeny of the genus Echinococcus has been a controversial issue for several years but with the usage of molecular tools to characterize the etiological agents these aspects had been clarified and a new classification has been proposed and is now widely accepted. The aim of this work is to ...
Natural polymorphisms of HIV type 2 pol sequences from drug-naive individuals
Parreira, R.; Monteiro, F.; Pádua, E.; Piedade, J.; Venenno, T.; Paixão, M.T.; Esteves, A.Until today, the susceptibility of human immunodeficiency virus type 2 (HIV-2) to protease and nucleosidic reverse-transcriptase inhibitors (PI and NRTI, respectively) has not been clearly documented. In this report we studied HIV-2 proviral sequences (n = 30) from drug-naive patients. Our results revealed that several amino acid positions in the protease and reverse transcriptase coding sequence harbored resid...
Spreading of HIV-1 subtype G and envB/gagG recombinant strains among injecting ...
Esteves, A; Parreira, R; Piedade, J; Venenno, T; Franco, M; Sousa, JG; Patrício, L; Brum, P; Costa, A; Canas-Ferreira, WWe have evaluated the genetic diversity of HIV-1 strains infecting injecting drug users (IDUs) in Lisbon, Portugal. Heteroduplex mobility assay and/or phylogenetic analysis revealed that env (C2V3C3 or gp41) subtype B is present in 63.7% of the 135 viral samples studied, followed by subtypes G (23.7%), A (6.7%), F (5.2%), and D (0.7%). Similar analysis of gag (p24/p7) performed on 91 of the specimens demonstrat...
Molecular epidemiology of HIV type 1 infection in Portugal: high prevalence of ...
Esteves, A; Parreira, R; Venenno, T; Franco, M; Piedade, J; Sousa, JG; Canas-Ferreira, WIn this study, we have investigated the diversity of current HIV-1 strains circulating in the metropolitan area of Lisbon, Portugal. A total of 217 HIV-1-positive blood samples, collected between October 1998 and December 2000, was genetically characterized in the gp120 C2V3C3 region (n = 205) or part of the gp41 N-terminal segment (n = 12) by heteroduplex mobility assay (HMA) and/or DNA sequencing. The HMA sub...
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