Lamotrigine analysis in blood and brain by high-performance liquid chromatography

• Author(s): Castel-Branco, M. M. ; Almeida, A. M. ; Falcão, A. C. ; Macedo, T. A. ; Caramona, M. M. ; Lopez, F. G.
• Date: 2001
• Persistent ID: http://hdl.handle.net/10316/5803
• Origin: Estudo Geral - Universidade de Coimbra
• Subject(s): Lamotrigine
• Document Type: Article
• Language: English

Description

A reversed-phase high-performance liquid chromatography assay was developed and validated to determine plasma and brain lamotrigine concentrations allowing pharmacokinetic-pharmacodynamic studies of this new antiepileptic drug in patients and laboratory animals. Lamotrigine and its internal standard were extracted, under alkaline conditions, from plasma and brain homogenate, into ethyl acetate; brain proteins were previously precipitated with trichloroacetic acid. The method was linear between 0.1 and 15.0 mg/l for plasma, with a detection limit of 0.008 mg/l, and between 0.1 and 5.0 mg/l for brain homogenate, with a detection limit of 0.023 mg/l. The method proved to be simple, useful and appropriate, not only for clinical and experimental research, but also for routine monitoring of lamotrigine concentrations in patients. http://www.sciencedirect.com/science/article/B6TG9-42T4DX1-G/1/114ff1c7668d24d37232dc1f255aeb16