Angiotensin II mediates catecholamine and neuropeptide Y secretion in human adr...

• Author(s): Cavadas, Cláudia ; Grand, Daniela ; Mosimann, François ; Cotrim, Maria Dulce ; Ribeiro, Carlos Alberto Fontes ; Brunner, Hans R. ; Grouzmann, Eric
• Date: 2003
• Persistent ID: http://hdl.handle.net/10316/5782
• Origin: Estudo Geral - Universidade de Coimbra
• Subject(s): Human adrenal chromaffin cells; Catecholamines; Neuropeptide Y; Angiotensin II; AT1 receptor; AT2 receptor
• Document Type: Article
• Language: English

Description

The aim of the present work was to study the effect of angiotensin II (Ang II) on catecholamines and neuropeptide Y (NPY) release in primary cultures of human adrenal chromaffin cells. Ang II stimulates norepinephrine (NE), epinephrine (EP) and NPY release from perifused chromaffin cells by 3-, 2- and 12-fold, respectively. The NPY release is more sustained than that of catecholamines. We found that the receptor-AT2 agonist, T2-(Ang II 4-8)2 has no effect on NE, EP and NPY release from chromaffin cells. We further showed that Ang II increases intracellular Ca2+ concentration ([Ca2+]i). The selective AT1-receptor antagonist Candesartan blocked [Ca2+]i increase by Ang II, while T2-(Ang II 4-8)2 was ineffective. These findings demonstrate that AT1 stimulation induces catecholamine secretion from human adrenal chromaffin cells probably by raising cytosolic calcium. http://www.sciencedirect.com/science/article/B6T0S-478J1GP-4/1/703d0edba8be6689cfd19851f6d304e7