Author(s):
Ferreira, F. M. L. 
; Palmeira, C. M. ; Seiça, R. ; Santos, M. S.
Date: 1999
Persistent ID: http://hdl.handle.net/10316/5461
Origin: Estudo Geral - Universidade de Coimbra
Document details
Alterations of liver mitochondrial bioenergetics in diabetic Goto-Kakizaki rats
Description
Respiratory indexes and the transmembrane electrical potential ([Delta][Psi]) were evaluated in mitochondrial preparations from 6-month-old Goto-Kakizaki (GK) and Wistar rats in the presence of glutamate + malate and succinate. We found that in diabetic GK mitochondria, flavin adenine dinucleotide (FAD)-linked respiratory indexes (respiratory control ratio [RCR] and adenosine diphosphate [ADP] to oxygen ratio [ADP/O]) are increased and uncoupled respiration is largely enhanced, indicating increased respiratory chain activity in GK rats. [Delta][Psi] development in GK mitochondrial preparations, energized using glutamate + malate or succinate as substrates, and the repolarization rate upon phosphorylation of the added ADP were significantly higher in GK mitochondrial preparations. These results indicate an enhanced activity of the phosphorylation system, confirmed by evaluating [Delta][Psi] development when the mitochondria are energized by adenosine triphosphate (ATP). Moreover, recovery of the potential upon a phosphorylative cycle is increased in GK mitochondria, reflecting a more efficient coupling between the phosphorylative and oxidative system. Contrasting with results obtained for alloxan- or streptozotocin-induced diabetic rats, this study clearly demonstrates no impairment of mitochondrial bioenergetics in diabetic GK rats. On the contrary, at this age, we observed a higher efficiency of the phosphorylation system as compared with Wistar rats. http://www.sciencedirect.com/science/article/B6WN4-4D6XNW8-Y5/1/286fd1697cd262bccbc2e10cd0d2bdbd
Respiratory indexes and the transmembrane electrical potential ([Delta][Psi]) were evaluated in mitochondrial preparations from 6-month-old Goto-Kakizaki (GK) and Wistar rats in the presence of glutamate + malate and succinate. We found that in diabetic GK mitochondria, flavin adenine dinucleotide (FAD)-linked respiratory indexes (respiratory control ratio [RCR] and adenosine diphosphate [ADP] to oxygen ratio [ADP/O]) are increased and uncoupled respiration is largely enhanced, indicating increased respiratory chain activity in GK rats. [Delta][Psi] development in GK mitochondrial preparations, energized using glutamate + malate or succinate as substrates, and the repolarization rate upon phosphorylation of the added ADP were significantly higher in GK mitochondrial preparations. These results indicate an enhanced activity of the phosphorylation system, confirmed by evaluating [Delta][Psi] development when the mitochondria are energized by adenosine triphosphate (ATP). Moreover, recovery of the potential upon a phosphorylative cycle is increased in GK mitochondria, reflecting a more efficient coupling between the phosphorylative and oxidative system. Contrasting with results obtained for alloxan- or streptozotocin-induced diabetic rats, this study clearly demonstrates no impairment of mitochondrial bioenergetics in diabetic GK rats. On the contrary, at this age, we observed a higher efficiency of the phosphorylation system as compared with Wistar rats. http://www.sciencedirect.com/science/article/B6WN4-4D6XNW8-Y5/1/286fd1697cd262bccbc2e10cd0d2bdbd
Document Type
Article
Language English
Language English
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