Autor(es):
Malfeito-Ferreira, Manuel 
; Loureiro, Virgilio ; Dias, L. ; Dias, S. ; Sancho, T. ; Stender, H. ; Querol, A.
Data: 2002
Identificador Persistente: http://hdl.handle.net/10400.5/490
Origem: Repositório da UTL
Assunto(s): 4-ethylphenol; yeast; wine
Descrição
www.elsevier.nl/locate/jnlabr/yfmic <p> DOI:10.1016/S0740-0020(02)00152-1 The ability to produce 4-ethylphenol from the substrate p-coumaric acid in synthetic media was evaluated for several yeast species
associated with wine production.Molar conversion rates as high as 90% were found by only Dekkera bruxellensis, D. anomala and
by some unidentified strains isolated from wine-related environments.Other unidentified strains produced traces of 4-ethylphenol.
All unidentified strains showed the same cultural characteristics as D. bruxellensis when grown on DBDM (Dekkera/Brettanomyces
differential medium) agar.The determination of long-chain fatty acid compositions and the utilization of peptide nucleic acid (PNA)
probes specific for D. bruxellensis showed that the unidentified strains did not belong to this species.Further identification, by
restriction pattern generated from PCR-amplification of the 5.8S rRNA gene and the two internal transcribed spacers (ITS),
assigned the unidentified strains to Candida cantarelli, C. wickerhamii, Debaryomyces hansenii, Kluyveromyces lactis and Pichia
guilliermondii.However, only some strains of P. guilliermondii were capable of converting p-coumaric acid into 4-ethylphenol with
efficiencies close to those observed in D. bruxellensis and D. anomala.
