Cloning, characterization, and tissue distribution of prolactin receptor in the...

• Author(s): Santos, Cecilia R. A. ; Ingleton, P. M. ; Cavaco, J. E. B. ; Kelly, P. A. ; Edery, M. ; Power, Deborah
• Date: 2001
• Persistent ID: http://hdl.handle.net/10400.1/5387
• Origin: Sapientia - Universidade do Algarve
• Subject(s): Prolactin receptor; Marine teleost; Osmoregulation; Reproduction
• Document Type: Article
• Language: English

Description

The prolactin receptor (PRLR) was cloned and its tissue distribution characterized in adults of the protandrous hermaphrodite marine teleost, the sea bream (Sparus aurata). An homologous cDNA probe for sea bream PRLR (sbPRLR) was obtained by RT-PCR using gill mRNA. This probe was used to screen intestine and kidney cDNA libraries from which two overlapping clones (1100 and 2425 bp, respectively) were obtained.