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Olfactory sensitivity to changes in environmental Ca2 in the freshwater teleost...
Hubbard, Peter; Ingleton, P. M.; Bendell, L. A.; Barata, E. N.; Canario, Adelino V. M.Olfactory sensitivity to changes in environmental Ca2+ has been demonstrated in two teleost species; a salmonid (Oncorhynchus nerka) and a marine/estuarine perciform (Sparus aurata). To assess whether this phenomenon is restricted to species that normally experience large fluctuations in external ion concentrations (e.g. moving from sea water to fresh water) or is present in a much wider range of species, we in...
Cloning, characterization, and tissue distribution of prolactin receptor in the...
Santos, Cecilia R. A.; Ingleton, P. M.; Cavaco, J. E. B.; Kelly, P. A.; Edery, M.; Power, DeborahThe prolactin receptor (PRLR) was cloned and its tissue distribution characterized in adults of the protandrous hermaphrodite marine teleost, the sea bream (Sparus aurata). An homologous cDNA probe for sea bream PRLR (sbPRLR) was obtained by RT-PCR using gill mRNA. This probe was used to screen intestine and kidney cDNA libraries from which two overlapping clones (1100 and 2425 bp, respectively) were obtained.
Immunochemical detection of parathyroid hormone-related protein in the saccus v...
Devlin, A. J.; Danks, J. A.; Faulkner, M. K.; Power, Deborah; Canario, Adelino V. M.; Martin, T. J.; Ingleton, P. M.Using antisera to regions of human parathyroid hormone- related protein (PTHrP) the saccus vasculosus (SV) of the sea bream (Sparus aurata) has been shown to contain immunoreactive PTHrP. By immunohistochemistry (IHC) the epithelial coronet cells in fixed and waxembedded SV tissue reacted with antisera to the prepro region of human PTHrP (−13 to +2), the N-terminus PTHrP (1–16), and the midmolecule PTHrP (50–69...
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