Author(s):
Almeida, Carina 
; Azevedo, N. F. ; Iversen, C. ; Fanning, S. ; Keevil, C. W. ; Vieira, M. J.
Date: 2009
Persistent ID: http://hdl.handle.net/1822/9454
Origin: RepositóriUM - Universidade do Minho
Document details
Development and application of a novel peptide nucleic acid Probe for the speci...
Description
Here, we report a fluorescence in situ hybridization (FISH) method for rapid detection of Cronobacter strains in powdered infant formula (PIF) using a novel peptide nucleic acid (PNA) probe. Laboratory tests with several Enterobacteriaceae species showed that the specificity and sensitivity of the method were 100%. FISH using PNA could detect as few as 1 CFU per 10 g of Cronobacter in PIF after an 8-h enrichment step, even in a mixed population containing bacterial contaminants.
Here, we report a fluorescence in situ hybridization (FISH) method for rapid detection of Cronobacter strains in powdered infant formula (PIF) using a novel peptide nucleic acid (PNA) probe. Laboratory tests with several Enterobacteriaceae species showed that the specificity and sensitivity of the method were 100%. FISH using PNA could detect as few as 1 CFU per 10 g of Cronobacter in PIF after an 8-h enrichment step, even in a mixed population containing bacterial contaminants.
Document Type
Article
Language English
Language English
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