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Detection of Escherichia coli O157 by Peptide Nucleic Acid Fluorescence In Situ...
Almeida, C.; Sousa, J. M.; Rocha, R.; Cerqueira, L.; Fanning, S.; Azevedo, N. F.; Vieira, M. J.Despite the emergence of non-O157 shiga toxin-producing Escherichia coli (STEC) infections, E. coli O157 serotype is still the most commonly identified STEC in world. It causes high morbidity and mortality, and has been responsible for a number of outbreaks in many parts of the world. Various methods have been developed to detect this particular serotype, but standard bacteriological methods remain as the gold ...
Development and application of a novel peptide nucleic acid Probe for the speci...
Almeida, Carina; Azevedo, N. F.; Iversen, C.; Fanning, S.; Keevil, C. W.; Vieira, M. J.Here, we report a fluorescence in situ hybridization (FISH) method for rapid detection of Cronobacter strains in powdered infant formula (PIF) using a novel peptide nucleic acid (PNA) probe. Laboratory tests with several Enterobacteriaceae species showed that the specificity and sensitivity of the method were 100%. FISH using PNA could detect as few as 1 CFU per 10 g of Cronobacter in PIF after an 8-h enrichmen...
Development and application of a novel Peptide Nucleic Acid probe for the speci...
Almeida, Carina; Azevedo, N. F.; Iversen, C.; Fanning, S.; Keevil, C. W.; Vieira, M. J.Cronobacter spp. are causative agents of meningitis, septicemia and necrotizing enterocolitis in neonates and immunocompromised infants. Recently, contaminated powdered infant formula (PIF) has been reported as a source of these infections. In order to minimize the risk of infection, the development of a rapid, sensitive and specific method for the early detection of this bacterium in infant formula is of the u...
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