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Development and application of a novel peptide nucleic acid Probe for the speci...
Almeida, Carina; Azevedo, N. F.; Iversen, C.; Fanning, S.; Keevil, C. W.; Vieira, M. J.Here, we report a fluorescence in situ hybridization (FISH) method for rapid detection of Cronobacter strains in powdered infant formula (PIF) using a novel peptide nucleic acid (PNA) probe. Laboratory tests with several Enterobacteriaceae species showed that the specificity and sensitivity of the method were 100%. FISH using PNA could detect as few as 1 CFU per 10 g of Cronobacter in PIF after an 8-h enrichmen...
Development and application of a novel Peptide Nucleic Acid probe for the speci...
Almeida, Carina; Azevedo, N. F.; Iversen, C.; Fanning, S.; Keevil, C. W.; Vieira, M. J.Cronobacter spp. are causative agents of meningitis, septicemia and necrotizing enterocolitis in neonates and immunocompromised infants. Recently, contaminated powdered infant formula (PIF) has been reported as a source of these infections. In order to minimize the risk of infection, the development of a rapid, sensitive and specific method for the early detection of this bacterium in infant formula is of the u...
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