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Detection of Salmonella enterica serovar Enteritidis using real time PCR, immun...

Several methods for the rapid and specific detection of Salmonella in food samples have been described. Here, we compare 4 of those methods in terms of assay time, procedure complexity, detection limit, sensitivity, specificity and accuracy. Milk, eggs and mayonnaise samples were artificially contaminated with Salmonella enterica serovar Enteritidis cell concentrations ranging from 1 × 10- 2 to 1 × 102 CFU per ...

Fluorescence in situ hybridization method using a peptide nucleic acid probe fo...

Lactobacillus species constitute one of the dominant and beneficial bacteria in our body and are used in developed countries as a microbial adjuvant. Identification of these probiotic bacteria is traditionally performed by culture-based techniques. However, such methods are very time-consuming and can give inaccurate results, especially when Lactobacillus is present in mixed bacterial complex communities. Our s...

Fluorescence in situ hybridization method using peptide nucleic acid probes for...

Background: Bacterial vaginosis (BV) is a common vaginal infection occurring in women of reproductive age. It is widely accepted that the microbial switch from normal microflora to BV is characterized by a decrease in vaginal colonization by Lactobacillus species together with an increase of Gardnerella vaginalis and other anaerobes. Our goal was to develop and optimize a novel Peptide Nucleic Acid (PNA) Fluore...

Rapid detection of urinary tract infections caused by Proteus spp. using PNA-FISH

We developed a fluorescence in situ hybridization (FISH) method for the rapid detection of Proteus spp. in urine, using a novel peptide nucleic acid (PNA) probe. Testing on 137 urine samples from patients with urinary tract infections has shown specificity and sensitivity values of 98 % (95 % CI, 93.2–99.7) and 100 % (95 % CI, 80,8–100), respectively, when compared with CHROMagar Orientation medium. Results ind...

Evaluation of the ability of C. albicans to form biofilm in the presence of pha...

Pseudomonas aeruginosa and Candida albicans are disparate microbial species, but both are known to be opportunistic pathogens frequently associated with nosocomial infections. The aim of this study was to provide a better understanding of the interactions between these microorganisms in dual-species biofilms. Several bacteriophage-resistant P. aeruginosa phenotypes have been isolated and were used in dual-speci...

Interaction between non-disease causing microorganism and E. coli in catheter-a...

Most biofilms involved in catheter-associated urinary tract infections (CAUTIs) are polymicrobial, with disease causing (e.g. E. coli) and non-disease causing (NDC) microorganisms (Delftia tsuruhstensis, Achromobacter xylosoxidans, Burkholderia fungorum) frequently co-inhabiting the same catheter. Nevertheless, there is a lack of knowledge about the role that NDC microorganisms have on biofilm formation by E. c...

Resistir ao inesperado: grupos de apoio: pais de crianças com NEE

O nascimento de uma criança com Necessidades Educativas Especiais (NEE) é um acontecimento inesperado na vida de uma família e de todos os seus elementos. Se no passado a atenção se centrava sobretudo na criança e na perturbação de que era portadora, na atualidade, a família, em especial os pais, são encarados como os principais intervenientes e promotores do processo de desenvolvimento da criança. O estágio re...

Proposal for a method to estimate nutrient shock effects in bacteria

Plating methods are still the golden standard in microbiology; however, some studies have shown that these techniques can underestimate the microbial concentrations and diversity. A nutrient shock is one of the mechanisms proposed to explain this phenomenon. In this study, a tentative method to assess nutrient shock effects was tested. Findings To estimate the extent of nutrient shock effects, two strains is...

Helicobacter pylori : role of water on survival and transmission

Biofilm formation on a microtiter-plate using artificial urine to mimic cathete...