Development and application of a novel peptide nucleic acid Probe for the speci...

• Autor(es): Almeida, Carina ; Azevedo, N. F. ; Iversen, C. ; Fanning, S. ; Keevil, C. W. ; Vieira, M. J.
• Data: 2009
• Identificador Persistente: http://hdl.handle.net/1822/9454
• Origem: RepositóriUM - Universidade do Minho
• Tipo de Documento: Artigo
• Idioma: Inglês

Descrição

Here, we report a fluorescence in situ hybridization (FISH) method for rapid detection of Cronobacter strains in powdered infant formula (PIF) using a novel peptide nucleic acid (PNA) probe. Laboratory tests with several Enterobacteriaceae species showed that the specificity and sensitivity of the method were 100%. FISH using PNA could detect as few as 1 CFU per 10 g of Cronobacter in PIF after an 8-h enrichment step, even in a mixed population containing bacterial contaminants.