Induction of phenolic compounds in Hypericum perforatum L. cells by colletotric...

• Autor(es): Conceição, Luís F. R. ; Ferreres, Federico ; Tavares, R. M. ; Dias, Alberto Carlos Pires
• Data: 2006
• Identificador Persistente: http://hdl.handle.net/1822/8933
• Origem: RepositóriUM - Universidade do Minho
• Assunto(s): Hypericum; Colletotrichum; Phenolics; Xanthone
• Tipo de Documento: Artigo
• Idioma: Inglês

Descrição

Changes in phenolic metabolism after elicitation with Colletotrichum gloeosporioides (CG) has been studied in Hypericum perforatum L. (HP) cell suspension cultures. Soluble phenolics were analysed by HPLC–DAD and HPLC–DAD–MS/MS. HP cultures elicited with the CG elicitor showed a significant increase in xanthone accumulation. Xanthone accumulation increased twelve fold when the cells were primed with methyl-jasmonate (MeJ) or salicylic acid (SA), before elicitation. HP cultures exposed only to MeJ produced a set of flavonoids, the flavones which represent a substantial part (approx. 40%) of the total flavonoids accumulated in these cells. The possible importance of xanthones as a component of defence mechanism of HP against biotic stress is discussed.