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Induction of phenolic compounds in Hypericum perforatum L. cells by colletotric...
Conceição, Luís F. R.; Ferreres, Federico; Tavares, R. M.; Dias, Alberto Carlos PiresChanges in phenolic metabolism after elicitation with Colletotrichum gloeosporioides (CG) has been studied in Hypericum perforatum L. (HP) cell suspension cultures. Soluble phenolics were analysed by HPLC–DAD and HPLC–DAD–MS/MS. HP cultures elicited with the CG elicitor showed a significant increase in xanthone accumulation. Xanthone accumulation increased twelve fold when the cells were primed with methyl-jasm...
Phytochemical and antioxidant characterization of Hypericum perforatum alcoholi...
Silva, Bruno A.; Ferreres, Federico; Malva, João O.; Dias, Alberto C. P.The antioxidant potentials of a total ethanolic extract of Hypericum perforatum (TE) and fractions were evaluated and correlated with their phenolic contents. The extracts were fully characterised by HPLC-DAD-MS-MS. Kaempferol 3-rutinoside and rutin-acetyl were identified for the first time in TE extracts. The free radical-scavenging properties of TE (EC50=21 [mu]g dwb/ml) and fractions were studied using DPPH....
Alterações na produção de compostos fenólicos em culturas de células de Vitis v...
Lima, M. R. M.; Guimarães, Orlando; Ferreres, Federico; Dias, Alberto Carlos PiresA esca é uma doença que afecta plantas da espécie Vitis vinifera levando a importantes perdas na produção de vinho. O fungo Phaeomoniella chlamydospora (PC) é frequentemente associado a plantas de videira com esca e declínio das vinhas. Informação sobre a interacção deste fungo com a esca e declínio das vinhas ainda é escassa. Para estudar mecanismos de defesa da videira ao fungo PC utilizamos culturas in vitro...
Differential phenolic production of Vitis vinifera cell cultures induced by Pha...
Lima, M. R. M.; Ferreres, Federico; Tavares, R. M.; Dias, Alberto Carlos PiresEsca is a destructive disease, of complex aetiology, that affects grapevines in several countries around the world, specially in the Mediterranean, South Africa and California (where is known as “black measles”). In the last few years the disease became a major concern given to its dramatic incidence increase. Till now, does not exist a method to treat plants attacked by Esca. To study the putative response of ...
Xanthone biosynthesis and accumulation in calli and suspended cells of Hypericu...
Dias, Alberto Carlos Pires; Seabra, Rosa M.; Andrade, Paula B.; Ferreres, Federico; Ferreira, Manuel FernandesCalli and suspended cells of Hypericum androsaemum accumulated high levels of 1,3,5,6 and 1,3,6,7 oxygenated xanthones. The major compounds include simple oxygenated xanthones or derivatives with prenyl, pyran or methoxyl groups, four of them being new natural compounds. A hypothetical biosynthetic scheme is proposed based on the isolated compounds and statistical analysis. Xanthone accumulation was influenced ...
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