Author(s):
Cerqueira, L. 
; Fernandes, R. M. ; Ferreira, Rui M. ; Oleastro, Mónica ; Carneiro, Fátima ; Brandão, Catarina ; Nunes, Pedro Pimentel ; Ribeiro, Mário Dinis ; Figueiredo, C. ; Keevil, C. W. ; Vieira, M. J. ; Azevedo, N. F.
Date: 2013
Persistent ID: http://hdl.handle.net/1822/27739
Origin: RepositóriUM - Universidade do Minho
Description
In this study an evaluation of Probe4Pylori, a previously established PNA-FISH
diagnostic test for H. pylori clarithromycin resistance in paraffin embedded gastric
biopsies was performed and compared against culture followed by E-test
and PCR. The Probe4Pylori showed very promising results with values of sensitivity
(80%) and specificity (approximately 90%) for the patients harboring
clarithromycin-resistant H. pylori. Due to the fact that different biopsies from
the same patient were used for culture and for molecular methods, the relatively
low value of sensitivity can be explained by the heterogeneous distribution
of H. pylori cells in the stomach, as for some patients only one of the
biopsies contained H. pylori. Furthermore, the results from Probe4Pylori did
not seem to be affected by previous treatments with antimicrobials and proton
pump inhibitors. It is also the only method tested here that allows direct visualization
of different H. pylori strains (resistant and susceptible). Future application
of this kit will hopefully engage the administration of more adequate
therapies to eradicate this bacterium.
