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Validation of a fluorescence in situ hybridization method using peptide nucleic...
Cerqueira, L.; Fernandes, R. M.; Ferreira, Rui M.; Oleastro, Mónica; Carneiro, Fátima; Brandão, Catarina; Nunes, Pedro Pimentel; Ribeiro, Mário DinisHere, we evaluated a previously established peptide nucleic acid-fluorescence in situ hybridization (PNA-FISH) method as a new diagnostic test for Helicobacter pylori clarithromycin resistance detection in paraffin-embedded gastric biopsy specimens. Both a retrospective study and a prospective cohort study were conducted to evaluate the specificity and sensitivity of a PNA-FISH method to determine H. pylori cla...
Probe4pylori : a new kit for the rapid detection of H. pylori and associated cl...
Cerqueira, L.; Fernandes, R. M.; Ferreira, Rui M.; Oleastro, Mónica; Carneiro, Fátima; Brandão, Catarina; Nunes, Pedro Pimentel; Ribeiro, Mário DinisIn this study an evaluation of Probe4Pylori, a previously established PNA-FISH diagnostic test for H. pylori clarithromycin resistance in paraffin embedded gastric biopsies was performed and compared against culture followed by E-test and PCR. The Probe4Pylori showed very promising results with values of sensitivity (80%) and specificity (approximately 90%) for the patients harboring clarithromycin-resistant H....
Influence of land use on nutrients transport using SWAT model: Study case of Ar...
DURÃO, A.; BRITO, D.; MORAIS, M.; FERNANDES, R.M.; NEVES, R.The impacts of agricultural activities in the environment are due to the lixiviation and soil erosion. Pollution of agricultural origin presents diffuse characteristics reaching the environment through surface runoff, lateral flow and groundwater flow. Models can contribute to understand the sources and the processes responsible for pollution, also identifying areas of concern. To evaluate the status of river w...
Estimation of transported pollutant load in Ardila catchment using the SWAT model
DURÃO, A.; LEITÃO, P.; BRITO, D.; FERNANDES, R.M.; NEVES, R.; MORAIS, M.Excess of organic matter and nutrients in the water body promotes algae blooms, which can accelerate the eutrophication process, situation often observed in the Ardila river. This river was identified as very polluted and classified as critical for Alqueva-Pedrogão System. The aim of this study was to estimate the transported nutrients load in a transboundary catchment using the SWAT (Soil and Water Assessment ...
PNA-FISH as a new diagnostic method for the determination of clarithromycin res...
Fernandes, R. M.; Cerqueira, L.; Ferreira, Rui M.; Carneiro, Fátima; Ribeiro, Mário Dinis; Figueiredo, C.; Keevil, C. W.; Azevedo, N. F.; Vieira, M. J.Triple therapy is the gold standard treatment for Helicobacter pylori eradication from the human stomach, but increased resistance to clarithromycin became the main factor of treatment failure. Until now, fastidious culturing methods are generally the method of choice to assess resistance status. In this study, a new genotypic method to detect clarithromycin resistance in clinical samples, based on fluorescent ...
Application of fluorescence in situ hybridisation using peptide nucleic acid pr...
Cerqueira, L.; Fernandes, R. M.; Ferreira, Rui M.; Carneiro, F.; Ribeiro, M. Dinis; Figueiredo, C.; Azevedo, N. F.; Keevil, C. W.; Vieira, M. J.Microorganisms are responsible for several infectious diseases that can cause severe problems to patients and their treatment success is seriously correlated with the fast detection of the infectious agent. Some of the standard methods used, such as culturing methods are fastidious and time-consuming and do not give any information about the antibiotic resistance profile. Therefore, molecular methods have been ...
Neotectónica e períodos de recorrência de grandes sismos e tsunamis na margem S...
Cunha, Tiago A.; Matias, Luís M.; Terrinha, Pedro; Negredo, A. M.; Rosas, Filipe; Fernandes, R. M. S.; Pinheiro, Luís M.Neste trabalho usámos o método numérico das placas finas (Bird, 1999) para modelar a Neotectónica no Golfo de Cadiz e estimar os períodos de recorrência de grandes sismos e tsunamis. Foram testadas várias configurações de falhas e condições fronteira, e os resultados comparados com as observações de GPS, tensão e deformação sísmica. O melhor ajuste às observações é obtido com um modelo que apresenta uma taxa...
Fluorescence in situ hybridization method using a peptide nucleic acid probe fo...
Almeida, Carina; Azevedo, N. F.; Fernandes, R. M.; Keevil, C. W.; Vieira, M. J.A fluorescence in situ hybridization (FISH) method for the rapid detection of Salmonella spp. using a novel peptide nucleic acid (PNA) probe was developed. Specificity and sensitivity probe matching theoretical estimates were both 100%. The PNA FISH method was optimized, and laboratory testing on representative strains from the Salmonella genus subspecies and several related bacterial species, confirmed the pre...
Oral Candida carriage of patients attending a dental clinic in Braga, Portugal
Martins, M.; Henriques, Mariana; Ribeiro, A. P.; Fernandes, R. M.; Gonçalves, Vânia; Seabra, A.; Azeredo, Joana; Oliveira, RosárioThe ability of the Candida species to colonize surfaces can be considered as a risk factor for oral infection. Aims The aim of this work was to establish oral Candida carriage in patients attending a dental clinic in Braga, Portugal. Methods A total of 97 patients were analysed. Swab samples were collected, and directly cultured onto CHROMagar Candida. Representative yeasts were identified by polymerase chain ...
Characterization of multispecies biofilms by peptide nucleic acid fluorescence ...
Almeida, Carina; Vieira, M. J.; Keevil, C. W.; Fernandes, R. M.; Azevedo, N. F.Our current understanding of biofilms in the environment and in health indicates that these structures are typically composed of many different microbial species. However, the lack of reliable techniques for the quantification, visualization and discrimination of each population has meant that studies assessing multi-species interactions between sessile microorganisms are scarce and low-throughput. Employing no...
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