Encontrados 13 documentos, a visualizar página 1 de 2
Validation of a fluorescence in situ hybridization method using peptide nucleic...
Cerqueira, L.; Fernandes, R. M.; Ferreira, Rui M.; Oleastro, Mónica; Carneiro, Fátima; Brandão, Catarina; Nunes, Pedro Pimentel; Ribeiro, Mário DinisHere, we evaluated a previously established peptide nucleic acid-fluorescence in situ hybridization (PNA-FISH) method as a new diagnostic test for Helicobacter pylori clarithromycin resistance detection in paraffin-embedded gastric biopsy specimens. Both a retrospective study and a prospective cohort study were conducted to evaluate the specificity and sensitivity of a PNA-FISH method to determine H. pylori cla...
Probe4pylori : a new kit for the rapid detection of H. pylori and associated cl...
Cerqueira, L.; Fernandes, R. M.; Ferreira, Rui M.; Oleastro, Mónica; Carneiro, Fátima; Brandão, Catarina; Nunes, Pedro Pimentel; Ribeiro, Mário DinisIn this study an evaluation of Probe4Pylori, a previously established PNA-FISH diagnostic test for H. pylori clarithromycin resistance in paraffin embedded gastric biopsies was performed and compared against culture followed by E-test and PCR. The Probe4Pylori showed very promising results with values of sensitivity (80%) and specificity (approximately 90%) for the patients harboring clarithromycin-resistant H....
Whole blood transfusion in small animals: indications and effects
Godinho-Cunha,Luís F; Ferreira,Rui M. R. F; Silvestre-Ferreira,Ana CTransfusion therapy is a major resource that can improve the patient's capability to overcome the underlying disease. However, the effects of whole blood infusion, and how they affect the patient's outcome, are not yet clear. For this study, a protocol was developed in order to monitor a group of 15 animals (9 dogs, 6 cats) that received a total of 19 transfusions; 3 animals received more than one transfusion e...
PNA-FISH as a new diagnostic method for the determination of clarithromycin res...
Fernandes, R. M.; Cerqueira, L.; Ferreira, Rui M.; Carneiro, Fátima; Ribeiro, Mário Dinis; Figueiredo, C.; Keevil, C. W.; Azevedo, N. F.; Vieira, M. J.Triple therapy is the gold standard treatment for Helicobacter pylori eradication from the human stomach, but increased resistance to clarithromycin became the main factor of treatment failure. Until now, fastidious culturing methods are generally the method of choice to assess resistance status. In this study, a new genotypic method to detect clarithromycin resistance in clinical samples, based on fluorescent ...
Application of fluorescence in situ hybridisation using peptide nucleic acid pr...
Cerqueira, L.; Fernandes, R. M.; Ferreira, Rui M.; Carneiro, F.; Ribeiro, M. Dinis; Figueiredo, C.; Azevedo, N. F.; Keevil, C. W.; Vieira, M. J.Microorganisms are responsible for several infectious diseases that can cause severe problems to patients and their treatment success is seriously correlated with the fast detection of the infectious agent. Some of the standard methods used, such as culturing methods are fastidious and time-consuming and do not give any information about the antibiotic resistance profile. Therefore, molecular methods have been ...
Helicobacter pylori colonization of the adenotonsillar tissue : fact or fiction?
Vilarinho, Sérgio; Guimarães, N.; Ferreira, Rui M.; Gomes, Bárbara; Wen, Xiaogang; Vieira, M. J.; Carneiro, Fátima; Godinho, Tiago; Figueiredo, C.Objective: The transmission of the gastric pathogen Helicobacter pylori involves the oral route. Molecular techniques have allowed the detection of H. pylori DNA in samples of the oral cavity, although culture of H. pylori from these type of samples has been sporadic. Studies have tried to demonstrate the presence of H. pylori in adenotonsillar tissue, with contradictory results. Our aim was to clarify whether ...
The thickness of the transport layer in stratified geomorphic flows
Leal, J. B.; Ferreira, Rui M. L.; Cardoso, António H.River Flow 2010 ; This paper is aimed at the development of a model for the thickness of the transport layer in stratified geomorphic flows such as sheet-flows or immature debris flows occurring, for instance, as a consequence of dam failure. These are flows with high geomorphic potential, occurring at or generating high shear stresses, whose ultimate driving mechanism is gravity. The micromechanical character...
Geomorphic dam-break flows. Part II: numerical simulation
Leal, J. G. A. B.; Ferreira, Rui M. L.; Cardoso, António H.Proceedings of the Institution of Civil Engineers - Water Management 163 Issue WM6
A conceptual model for sheet-flow drawn from rapid granular flow theories
Leal, J. B.; Ferreira, Rui M. L.; Nicolau, Vanessa A.; Amaral, Sílvia R.; Almeida, António B.33rd IAHR Congress: Water Engineering for a Sustainable Environment ; This paper is aimed at presenting i) a simple, yet sound, conceptual model applicable to the simulation of erosion, deposition and transport of cohesionless sediment in stratified flows under high shear stresses and ii) numerical solutions in idealized unsteady flow non-equilibrium transport situations. The conceptual model for the granular ...
Organised turbulence over mobile and immobile hydraulically rough boundaries
Leal, J. B.; Ferreira, Rui M. L.; Franca, Mário J.; Cardoso, António H.33rd IAHR Congress: Water Engineering for a Sustainable Environment ; The present work is aimed at the study of near-bed organised turbulence over mobile and immobile, porous, hydraulically rough boundaries. The bed was permeable and composed of non-cohesive sediments. Two data sets were analysed, characterised by the same u*. The mobile bed data featured generalised sediment transport for all size fractions s...
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