Encontrados 7 documentos, a visualizar página 1 de 1
Validation of a fluorescence in situ hybridization method using peptide nucleic...
Cerqueira, L.; Fernandes, R. M.; Ferreira, Rui M.; Oleastro, Mónica; Carneiro, Fátima; Brandão, Catarina; Nunes, Pedro Pimentel; Ribeiro, Mário DinisHere, we evaluated a previously established peptide nucleic acid-fluorescence in situ hybridization (PNA-FISH) method as a new diagnostic test for Helicobacter pylori clarithromycin resistance detection in paraffin-embedded gastric biopsy specimens. Both a retrospective study and a prospective cohort study were conducted to evaluate the specificity and sensitivity of a PNA-FISH method to determine H. pylori cla...
Probe4pylori : a new kit for the rapid detection of H. pylori and associated cl...
Cerqueira, L.; Fernandes, R. M.; Ferreira, Rui M.; Oleastro, Mónica; Carneiro, Fátima; Brandão, Catarina; Nunes, Pedro Pimentel; Ribeiro, Mário DinisIn this study an evaluation of Probe4Pylori, a previously established PNA-FISH diagnostic test for H. pylori clarithromycin resistance in paraffin embedded gastric biopsies was performed and compared against culture followed by E-test and PCR. The Probe4Pylori showed very promising results with values of sensitivity (80%) and specificity (approximately 90%) for the patients harboring clarithromycin-resistant H....
PNA-FISH as a new diagnostic method for the determination of clarithromycin res...
Fernandes, R. M.; Cerqueira, L.; Ferreira, Rui M.; Carneiro, Fátima; Ribeiro, Mário Dinis; Figueiredo, C.; Keevil, C. W.; Azevedo, N. F.; Vieira, M. J.Triple therapy is the gold standard treatment for Helicobacter pylori eradication from the human stomach, but increased resistance to clarithromycin became the main factor of treatment failure. Until now, fastidious culturing methods are generally the method of choice to assess resistance status. In this study, a new genotypic method to detect clarithromycin resistance in clinical samples, based on fluorescent ...
Mixed lineage kinase 3 gene mutations in mismatch repair deficient gastrointest...
Velho, Sérgia; Oliveira, Carla; Paredes, Joana; Sousa, Sónia; Leite, Marina; Matos, Paulo; Milanezi, Fernanda; Ribeiro, Ana Sofia; Mendes, NunoMixed lineage kinase 3 (MLK3) is a serine/threonine kinase, regulating MAPkinase signalling, in which cancer-associated mutations have never been reported. In this study, 174 primary gastrointestinal cancers (48 hereditary and 126 sporadic forms) and 7 colorectal cancer cell lines were screened for MLK3 mutations. MLK3 mutations were significantly associated with MSI phenotype in primary tumours (P = 0.0005), o...
Helicobacter pylori colonization of the adenotonsillar tissue : fact or fiction?
Vilarinho, Sérgio; Guimarães, N.; Ferreira, Rui M.; Gomes, Bárbara; Wen, Xiaogang; Vieira, M. J.; Carneiro, Fátima; Godinho, Tiago; Figueiredo, C.Objective: The transmission of the gastric pathogen Helicobacter pylori involves the oral route. Molecular techniques have allowed the detection of H. pylori DNA in samples of the oral cavity, although culture of H. pylori from these type of samples has been sporadic. Studies have tried to demonstrate the presence of H. pylori in adenotonsillar tissue, with contradictory results. Our aim was to clarify whether ...
DNA copy number profiles of gastric cancer precursor lesions
Buffart, Tineke; Carvalho, Beatriz; Mons, Thomas; Reis, R. M.; Moutinho, Cátia; Silva, Paula; Grieken, Nicole C. T. van; Vieth, Michael; Stolte, ManfredChromosomal instability (CIN) is the most prevalent type of genomic instability in gastric tumours, but its role in malignant transformation of the gastric mucosa is still obscure. In the present study, we set out to study whether two morphologically distinct categories of gastric cancer precursor lesions, i.e. intestinal-type and pyloric gland adenomas, would carry different patterns of DNA copy number changes...
E-Cadherin (CDH1) and p53 rather than SMAD4 and Caspase-10 germline mutations c...
Oliveira, Carla; Ferreira, Paulo; Nabais, Sérgio; Campos, Luísa; Ferreira, Ana; Cirnes, Luís; Alves, Catarina Castro; Veiga, Isabel; Fragoso, MariaApproximately 30% of all hereditary diffuse gastric cancer (HDGC) families carry CDH1 germline mutations. The other two thirds remain genetically unexplained and are probably caused by alterations in other genes. Using polymerase chain reaction (PCR)/single-strand conformation polymorphism (SSCP)/sequencing, we screened 32 Portuguese families with a history of gastric cancer and 23 patients with early onset gas...
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