Descrição
Microbial cellulase production has attracted great attention due its
several applications. In this context, studies that become this practice feasible are
very important. Corncob is an inexpensive byproduct, which contains more than
30% of cellulose; however, this material is not readily available to enzymatic
hydrolysis and pretreatment of lignocellulosic material in autohydrolysis
processes become this more easy. The inclusion of this pretreated material in the
nutrient media can be a strategy to increase and undervalue cellulase production.
The best pretreatment conditions for total cellulase (FPase), β-glucosidase and
exoglucanase (avicelase) were obtained using the solid fraction pretreated at
200ºC for 30 or 50 minutes. It was not detected endoglucanase production
(CMCase) using corncob without treatment. However, its induction was observed
when T. reesei was cultivated with the corncob liquid fraction obtained after 30
minutes of pretreatment, mainly at 200ºC, where the production was maximal.