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Pré-tratamento de resíduos agro-industriais e novas perspectivas na produção de...

Freitas, S. S.; Brazil, O. A. Vital; Nascimento, M. B.; Ferreira, L. F. Romanholo; Gonçalves, A. R.; Michelin, Michele; Polizeli, Maria de Lourdes T. M.

Visando contribuir para a melhoria das condições ambientais pela eliminação dos resíduos agrícolas/agroindustriais, bem como proporcionar a formação de uma linha de pesquisa com propósito de captar e disseminar informações técnicas e tecnológicas agregando maior valor nestes subprodutos ou resíduos é que o presente trabalho foi realizado. Deste modo, o sabugo de milho in natura foi submetido a dois diferentes p...


Autohydrolysis pretreatment of corncob for cellulase production by Trichoderma ...

Michelin, Michele; Ruzene, Denise S.; Silva, Daniel Pereira da; Vicente, A. A.; Jorge, João A.; Teixeira, J. A.; Polizeli, Maria de Lourdes T. M.

Microbial cellulase production has attracted great attention due its several applications. In this context, studies that become this practice feasible are very important. Corncob is an inexpensive byproduct, which contains more than 30% of cellulose; however, this material is not readily available to enzymatic hydrolysis and pretreatment of lignocellulosic material in autohydrolysis processes become this more e...


Evaluation of autohydrolysis process for cellulases production by Aspergillus n...

Michelin, Michele; Ruzene, Denise S.; Silva, Daniel Pereira da; Vicente, A. A.; Teixeira, J. A.; Polizeli, Maria de Lourdes T. M.


Xylanase and β-xylosidase production by Aspergillus ochraceus : new perspective...

Michelin, Michele; Polizeli, Maria de Lourdes T. M.; Ruzene, Denise S.; Silva, Daniel Pereira da; Vicente, A. A.; Jorge, João A.; Terenzi, Héctor F.

The xylanase biosynthesis is induced by its substrate—xylan. The high xylan content in some wastes such as wheat residues (wheat bran and wheat straw) makes them accessible and cheap sources of inducers to be mainly applied in great volumes of fermentation, such as those of industrial bioreactors. Thus, in this work, the main proposal was incorporated in the nutrient medium wheat straw particles decomposed to s...


Bioethanol production from hydrothermal pretreated wheat straw by a flocculatin...

Ruíz, Héctor A.; Silva, Daniel P.; Ruzene, Denise S.; Lima, Luís; Vicente, A. A.; Teixeira, J. A.

Wheat straw is nowadays being considered a potential lignocellulose raw material for fuel ethanol production of second generation and as an alternative to conventional fuel ethanol production from cereal crops. In the present study, hydrothermal pretreated wheat straw with high cellulose content (>60%) at 180 °C for 30 min was used as substrate in simultaneous saccharification and fermentation (SSF) process for...


Production of xylanase and β-xylosidase from autohydrolysis liquor of corncob u...

Michelin, Michele; Polizeli, Maria de Lourdes T. M.; Ruzene, Denise S.; Silva, Daniel Pereira da; Ruiz, Héctor A.; Vicente, A. A.; Jorge, João A.

Agroindustrial residues are materials often rich in cellulose and hemicellulose. The use of these substrates for the microbial production of enzymes of industrial interest is mainly due to their high availability associated with their low cost. In this work, corncob (CCs) particles decomposed to soluble compounds (liquor) were incorporated in the microbial growth medium through autohydrolysis, as a strategy to ...


Evaluation of a hydrothermal process for pretreatment of wheat straw : effect o...

Ruiz, Héctor A.; Ruzene, Denise S.; Silva, Daniel Pereira da; Quintas, Mafalda A. C.; Vicente, A. A.; Teixeira, J. A.

Hydrothermal processes are an eco-friendly processes that provide an interesting alternative for chemical utilization of lignocellulosic materials, in which water and crop residues are the only reagents. In this work the effect of process conditions (size distribution of the wheat straw, temperature and time) was evaluated against production of fermentable products. RESULTS: The use of milled wheat straw fracti...


Development and characterization of an environmentallyfriendly process sequence...

Ruiz, Héctor A.; Ruzene, Denise S.; Silva, Daniel P.; Silva, Fernando F. Macieira da; Vicente, A. A.; Teixeira, J. A.

The present work describes the delignification of wheat straw through an environmentally friendly process resulting from sequential application of autohydrolysis and organosolv processes. Wheat straw autohydrolysis was performed at 180°C during 30 min with a liquid–solid ratio of 10 (v/w); under these conditions, a solubilization of 44% of the original xylan, with 78% of sugars as xylooligosaccharides of the su...


Production of xylanolytic enzymes by Aspergillus terricola in stirred tank and ...

Michelin, Michele; Polizeli, Maria de Lourdes T. M.; Ruzene, Denise S.; Silva, D. P.; Vicente, A. A.; Jorge, João A.; Terenzi, Héctor F.

Fungi producing high xylanase levels have attracted considerable attention because of their potential industrial applications. Batch cultivations of Aspergillus terricola fungus were evaluated in stirred tank and airlift bioreactors, by using wheat bran particles suspended in the cultivation medium as substrate for xylanase and b-xylosidase production. In the stirred tank bioreactor, in physical conditions of 3...


Pretreatment of brewers' spent grains for cellulases production by Aspergillus ...

Michelin, Michele; Polizeli, Maria de Lourdes T. M.; Ruzene, Denise S.; Silva, Daniel Pereira da; Vicente, A. A.; Teixeira, J. A.

Successful utilization of cellulosic materials as a renewable carbon source is dependent on the development of economically feasible process technologies both for the production of biomass-degrading enzymes, and for the enzymatic hydrolysis of cellulosic materials to low molecular weight products. Significant cost reduction is required in order to enhance the commercial viability of cellulase production technol...


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    Financiadores do RCAAP

Fundação para a Ciência e a Tecnologia Universidade do Minho   Governo Português Ministério da Educação e Ciência Programa Operacional da Sociedade do Conhecimento União Europeia