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Autohydrolysis pretreatment of corncob for cellulase production by Trichoderma ...

Michelin, Michele; Ruzene, Denise S.; Silva, Daniel Pereira da; Vicente, A. A.; Jorge, João A.; Teixeira, J. A.; Polizeli, Maria de Lourdes T. M.

Microbial cellulase production has attracted great attention due its several applications. In this context, studies that become this practice feasible are very important. Corncob is an inexpensive byproduct, which contains more than 30% of cellulose; however, this material is not readily available to enzymatic hydrolysis and pretreatment of lignocellulosic material in autohydrolysis processes become this more e...


Influence of volumetric oxygen transfer coefficient (kLa) on xylanases batch pr...

Michelin, Michele; Mota, A.; Polizeli, Maria de Lourdes T. M.; Silva, Daniel Pereira da; Vicente, A. A.; Teixeira, J. A.

Oxygenation is an important parameter involved in the design and operation of mixing-sparging bioreactors and it can be analyzed by means of the oxygen mass transfer coefficient (kLa). In this study, batch fermentations in stirred tank bioreactor (STB) and airlift bioreactor (ALB) were operated under a range of kLa values, in attempts to optimize and compare the activities of extracellular xylanase and β-xylosi...


Evaluation of autohydrolysis process for cellulases production by Aspergillus n...

Michelin, Michele; Ruzene, Denise S.; Silva, Daniel Pereira da; Vicente, A. A.; Teixeira, J. A.; Polizeli, Maria de Lourdes T. M.


Xylanase and β-xylosidase production by Aspergillus ochraceus : new perspective...

Michelin, Michele; Polizeli, Maria de Lourdes T. M.; Ruzene, Denise S.; Silva, Daniel Pereira da; Vicente, A. A.; Jorge, João A.; Terenzi, Héctor F.

The xylanase biosynthesis is induced by its substrate—xylan. The high xylan content in some wastes such as wheat residues (wheat bran and wheat straw) makes them accessible and cheap sources of inducers to be mainly applied in great volumes of fermentation, such as those of industrial bioreactors. Thus, in this work, the main proposal was incorporated in the nutrient medium wheat straw particles decomposed to s...


Production of xylanase and β-xylosidase from autohydrolysis liquor of corncob u...

Michelin, Michele; Polizeli, Maria de Lourdes T. M.; Ruzene, Denise S.; Silva, Daniel Pereira da; Ruiz, Héctor A.; Vicente, A. A.; Jorge, João A.

Agroindustrial residues are materials often rich in cellulose and hemicellulose. The use of these substrates for the microbial production of enzymes of industrial interest is mainly due to their high availability associated with their low cost. In this work, corncob (CCs) particles decomposed to soluble compounds (liquor) were incorporated in the microbial growth medium through autohydrolysis, as a strategy to ...


Evaluation of a hydrothermal process for pretreatment of wheat straw : effect o...

Ruiz, Héctor A.; Ruzene, Denise S.; Silva, Daniel Pereira da; Quintas, Mafalda A. C.; Vicente, A. A.; Teixeira, J. A.

Hydrothermal processes are an eco-friendly processes that provide an interesting alternative for chemical utilization of lignocellulosic materials, in which water and crop residues are the only reagents. In this work the effect of process conditions (size distribution of the wheat straw, temperature and time) was evaluated against production of fermentable products. RESULTS: The use of milled wheat straw fracti...


Pretreatment of brewers' spent grains for cellulases production by Aspergillus ...

Michelin, Michele; Polizeli, Maria de Lourdes T. M.; Ruzene, Denise S.; Silva, Daniel Pereira da; Vicente, A. A.; Teixeira, J. A.

Successful utilization of cellulosic materials as a renewable carbon source is dependent on the development of economically feasible process technologies both for the production of biomass-degrading enzymes, and for the enzymatic hydrolysis of cellulosic materials to low molecular weight products. Significant cost reduction is required in order to enhance the commercial viability of cellulase production technol...


Banana as adjunct in beer production : applicability and performance of ferment...

Carvalho, Giovani B. M.; Silva, Daniel Pereira da; Bento, Camila V.; Vicente, A. A.; Teixeira, J. A.; Felipe, Maria das Graças A.

Traditionally, the raw materials for beer production are barley, hops, water, and yeast, but most brewers use also different adjuncts. During the alcoholic fermentation, the contribution of aroma compounds from other ingredients to the final beer flavor depends on the wort composition, on the yeast strain, and mainly on the process conditions. In this context, banana can also be a raw material favorable to alco...


Análise da fermentação em bioreatores cilindrocônicos de bancada na elaboração ...

Matos, Gislaine Fernandes de; Teixeira, J. A.; Silva, Daniel Pereira da; Vicente, A. A.; Felipe, Maria das Graças A.; Silva, João B. Almeida e

O aumento do extrato inicial de fermentação e uso de adjuntos na substituição do malte vem sendo uma alternativa para diminuição de custos na produção de cervejas. Uma outra característica deste processo tem sido a utilização de nutrientes para aumentar a produtividade volumétrica em etanol. Pelo fato da banana ser uma fruta abundante no Brasil e ser pouco qualificada para a exportação, pode-se utilizá-la como ...


Application of wheat straw autohydrolysis liquor to xylanase and β-xylosidase l...

Michelin, Michele; Polizeli, Maria de Lourdes T. M.; Silva, Daniel Pereira da; Ruzene, Denise S.; Vicente, A. A.; Jorge, João A.; Terenzi, Héctor F.

Research into microbial xylanases production has increased due to its several applications. In this context, studies that make this practice feasible are important. Wheat bran is an inexpensive byproduct, which contains around 28% hemicellulose; however, the wheat bran particles suspended in the cultivation medium have to be decomposed to soluble compounds to be used by the fungi and the treatment of lignocellu...


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    Financiadores do RCAAP

Fundação para a Ciência e a Tecnologia Universidade do Minho   Governo Português Ministério da Educação e Ciência Programa Operacional da Sociedade do Conhecimento União Europeia